Sour cherry seed kernel extract increases heme oxygenase-1 expression and decreases representation of CD3+ TNF-α + and CD3 + IL-8+ subpopulations in peripheral blood leukocyte cultures from type 2 diabetes patients

Fadia F. Mahmoud, Rana Al-Awadhi, David D. Haines, Ali Dashti, Hussain Dashti, Ebaa Al-Ozairi, I. Bak, A. Tósaki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The present study evaluates a hypothesis that sour cherry (Prunus cerasus) seed extracts (SCE) modulate CD3+ T lymphocyte activity in ways predictive of potential for uses of SCE in management of inflammatory diseases. Peripheral blood mononuclear cells (PBMC) from 12 type 2 diabetes (T2DM) patients and eight healthy control subjects were cultured 24 h with 100 ng/ml lipopolysaccharide (LPS) to increase inflammatory signaling and co-incubated with 0.5-100 μg/ml SCE. Cultures were evaluated by two-color flow cytometry for percent representation of CD3+ IL8+ and CD3 + TNF-α + cells which express interleukin-8 (IL-8), and tumor necrosis factor-α, (TNF-α+) respectively, and by enzyme-linked immunoassay for lymphocyte-associated heme oxygenase-1 (HO-1, known to be induced by SCE). SCE dosage ranges of 0.5-100 μg/ml in cell cultures significantly suppressed LPS-increased CD3 + TNF-α + and CD3 + IL8+ representation from all participants (p <0.05), with greater pharmacological effect noted in suppression of CD3 + TNF-α + noted in cells from T2DM patients versus healthy control subjects. These effects correlated with increased HO-1 expression in SCE-treated PBMC from all subjects (p <0.05). Since TNF-α and IL-8 are diagnostic/prognostic biomarkers for many inflammatory syndromes, the capacity of SCE to down-regulate representation of cells that express them suggests potential for therapeutic use of SCE in T2DM and other diseases.

Original languageEnglish
Pages (from-to)767-774
Number of pages8
JournalPhytotherapy Research
Volume27
Issue number5
DOIs
Publication statusPublished - May 2013

Fingerprint

Heme Oxygenase-1
Interleukin-8
Type 2 Diabetes Mellitus
Seeds
Leukocytes
Lipopolysaccharides
Blood Cells
Healthy Volunteers
Prunus avium
Therapeutic Uses
Disease Management
Immunoenzyme Techniques
Flow Cytometry
Down-Regulation
Color
Cell Culture Techniques
Tumor Necrosis Factor-alpha
Biomarkers
Pharmacology
Lymphocytes

Keywords

  • diabetes
  • heme oxygenase-1
  • IL-8
  • inflammation
  • sour cherry
  • TNF-α

ASJC Scopus subject areas

  • Pharmacology

Cite this

Sour cherry seed kernel extract increases heme oxygenase-1 expression and decreases representation of CD3+ TNF-α + and CD3 + IL-8+ subpopulations in peripheral blood leukocyte cultures from type 2 diabetes patients. / Mahmoud, Fadia F.; Al-Awadhi, Rana; Haines, David D.; Dashti, Ali; Dashti, Hussain; Al-Ozairi, Ebaa; Bak, I.; Tósaki, A.

In: Phytotherapy Research, Vol. 27, No. 5, 05.2013, p. 767-774.

Research output: Contribution to journalArticle

@article{3492bbda55284dc392f166f4fd760c38,
title = "Sour cherry seed kernel extract increases heme oxygenase-1 expression and decreases representation of CD3+ TNF-α + and CD3 + IL-8+ subpopulations in peripheral blood leukocyte cultures from type 2 diabetes patients",
abstract = "The present study evaluates a hypothesis that sour cherry (Prunus cerasus) seed extracts (SCE) modulate CD3+ T lymphocyte activity in ways predictive of potential for uses of SCE in management of inflammatory diseases. Peripheral blood mononuclear cells (PBMC) from 12 type 2 diabetes (T2DM) patients and eight healthy control subjects were cultured 24 h with 100 ng/ml lipopolysaccharide (LPS) to increase inflammatory signaling and co-incubated with 0.5-100 μg/ml SCE. Cultures were evaluated by two-color flow cytometry for percent representation of CD3+ IL8+ and CD3 + TNF-α + cells which express interleukin-8 (IL-8), and tumor necrosis factor-α, (TNF-α+) respectively, and by enzyme-linked immunoassay for lymphocyte-associated heme oxygenase-1 (HO-1, known to be induced by SCE). SCE dosage ranges of 0.5-100 μg/ml in cell cultures significantly suppressed LPS-increased CD3 + TNF-α + and CD3 + IL8+ representation from all participants (p <0.05), with greater pharmacological effect noted in suppression of CD3 + TNF-α + noted in cells from T2DM patients versus healthy control subjects. These effects correlated with increased HO-1 expression in SCE-treated PBMC from all subjects (p <0.05). Since TNF-α and IL-8 are diagnostic/prognostic biomarkers for many inflammatory syndromes, the capacity of SCE to down-regulate representation of cells that express them suggests potential for therapeutic use of SCE in T2DM and other diseases.",
keywords = "diabetes, heme oxygenase-1, IL-8, inflammation, sour cherry, TNF-α",
author = "Mahmoud, {Fadia F.} and Rana Al-Awadhi and Haines, {David D.} and Ali Dashti and Hussain Dashti and Ebaa Al-Ozairi and I. Bak and A. T{\'o}saki",
year = "2013",
month = "5",
doi = "10.1002/ptr.4783",
language = "English",
volume = "27",
pages = "767--774",
journal = "Phytotherapy Research",
issn = "0951-418X",
publisher = "John Wiley and Sons Ltd",
number = "5",

}

TY - JOUR

T1 - Sour cherry seed kernel extract increases heme oxygenase-1 expression and decreases representation of CD3+ TNF-α + and CD3 + IL-8+ subpopulations in peripheral blood leukocyte cultures from type 2 diabetes patients

AU - Mahmoud, Fadia F.

AU - Al-Awadhi, Rana

AU - Haines, David D.

AU - Dashti, Ali

AU - Dashti, Hussain

AU - Al-Ozairi, Ebaa

AU - Bak, I.

AU - Tósaki, A.

PY - 2013/5

Y1 - 2013/5

N2 - The present study evaluates a hypothesis that sour cherry (Prunus cerasus) seed extracts (SCE) modulate CD3+ T lymphocyte activity in ways predictive of potential for uses of SCE in management of inflammatory diseases. Peripheral blood mononuclear cells (PBMC) from 12 type 2 diabetes (T2DM) patients and eight healthy control subjects were cultured 24 h with 100 ng/ml lipopolysaccharide (LPS) to increase inflammatory signaling and co-incubated with 0.5-100 μg/ml SCE. Cultures were evaluated by two-color flow cytometry for percent representation of CD3+ IL8+ and CD3 + TNF-α + cells which express interleukin-8 (IL-8), and tumor necrosis factor-α, (TNF-α+) respectively, and by enzyme-linked immunoassay for lymphocyte-associated heme oxygenase-1 (HO-1, known to be induced by SCE). SCE dosage ranges of 0.5-100 μg/ml in cell cultures significantly suppressed LPS-increased CD3 + TNF-α + and CD3 + IL8+ representation from all participants (p <0.05), with greater pharmacological effect noted in suppression of CD3 + TNF-α + noted in cells from T2DM patients versus healthy control subjects. These effects correlated with increased HO-1 expression in SCE-treated PBMC from all subjects (p <0.05). Since TNF-α and IL-8 are diagnostic/prognostic biomarkers for many inflammatory syndromes, the capacity of SCE to down-regulate representation of cells that express them suggests potential for therapeutic use of SCE in T2DM and other diseases.

AB - The present study evaluates a hypothesis that sour cherry (Prunus cerasus) seed extracts (SCE) modulate CD3+ T lymphocyte activity in ways predictive of potential for uses of SCE in management of inflammatory diseases. Peripheral blood mononuclear cells (PBMC) from 12 type 2 diabetes (T2DM) patients and eight healthy control subjects were cultured 24 h with 100 ng/ml lipopolysaccharide (LPS) to increase inflammatory signaling and co-incubated with 0.5-100 μg/ml SCE. Cultures were evaluated by two-color flow cytometry for percent representation of CD3+ IL8+ and CD3 + TNF-α + cells which express interleukin-8 (IL-8), and tumor necrosis factor-α, (TNF-α+) respectively, and by enzyme-linked immunoassay for lymphocyte-associated heme oxygenase-1 (HO-1, known to be induced by SCE). SCE dosage ranges of 0.5-100 μg/ml in cell cultures significantly suppressed LPS-increased CD3 + TNF-α + and CD3 + IL8+ representation from all participants (p <0.05), with greater pharmacological effect noted in suppression of CD3 + TNF-α + noted in cells from T2DM patients versus healthy control subjects. These effects correlated with increased HO-1 expression in SCE-treated PBMC from all subjects (p <0.05). Since TNF-α and IL-8 are diagnostic/prognostic biomarkers for many inflammatory syndromes, the capacity of SCE to down-regulate representation of cells that express them suggests potential for therapeutic use of SCE in T2DM and other diseases.

KW - diabetes

KW - heme oxygenase-1

KW - IL-8

KW - inflammation

KW - sour cherry

KW - TNF-α

UR - http://www.scopus.com/inward/record.url?scp=84876465673&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876465673&partnerID=8YFLogxK

U2 - 10.1002/ptr.4783

DO - 10.1002/ptr.4783

M3 - Article

C2 - 22848037

AN - SCOPUS:84876465673

VL - 27

SP - 767

EP - 774

JO - Phytotherapy Research

JF - Phytotherapy Research

SN - 0951-418X

IS - 5

ER -