Some sources of error in the one-stage assay of factor VIII

Susan Elödi, Katalin Váradi, Susan R. Hollán

Research output: Contribution to journalArticle

11 Citations (Scopus)


The error of the one-stage assay of factor VIII activity is determined by the quality of reference and substrate (factor VIII-deficient) plasmas, as well as by the accuracy of dilution and clot observation time. Storage of the fresh normal plasma used as reference and/or of the substrate plasma for 2-3 h at 0°C may cause considerable errors, which can be eliminated by the immediate use of plasma or by the determination of an appropriate correction factor. With all precautions taken into account the average error of the assay in our hands is 20%.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalPathophysiology of Haemostasis and Thrombosis
Issue number1
Publication statusPublished - Jan 1 1978


  • Coagulation
  • Factor VIII assay
  • Haemophilia

ASJC Scopus subject areas

  • Hematology
  • Physiology (medical)

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