Some morphological and histochemical features of the midgut myenteric plexus of the common European frog, Rana esculenta

R. Gábriel, E. Fekete, M. Csoknya

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Abstract

The neuron morphology and distribution of four putative transmitters were investigated in the myenteric plexus of frog (Rana esculenta) midgut. The gross morphology was revealed by NADH-diaphorase histochemistry, and the shape of the neurons by silver impregnation. Nerve cells had heterogeneous distribution: they either formed ganglia or placed as solitary neurons in the duodenum, while in the rest of the midgut only solitary neurons were observed. Three morphologically distinct cell types were revealed by silver impregnation: mainly type I and type II neurons cells were seen in the duodenum, while the rest of the intestine contained type II and III cells. Catecholamine fluorescence was revealed in nerve fibres in the duodenum, while few small nerve cells were observed in the small intestinal region. Acetylcholinesterase histochemistry showed strongly reactive nerve cells that were associated with the main fibre bundles in the duodenum. Only longitudinally oriented fibres and occasionally stained neurons were seen in the small intestine. Substance P immunocytochemistry revealed an extensive plexus, which contained a moderate number of stained perikarya in the full length of the midgut. Gamma-aminobutyric acid showed non-uniform distribution in the two parts of the midgut: a stronger and more regular fibre staining was found in the duodenum then in the rest of the intestine. Ultrastructural observations demonstrated that intrinsic neurons received synaptic inputs from the profiles contained agranular vesicles, while 'P'-type profiles established close contacts with neurons. Both profile types formed close contacts with the smooth muscle cells. In spite of the several neuromorphological and ultrastructural similarities to the mammalian species, the transmitter distribution described here does not match in all respects to that found in the enteric nervous system of those animals. This finding reflects the view, that the neurochemical coding system and the projection pattern within the plexus may vary considerably among different classes of vertebrates.

Original languageEnglish
Pages (from-to)83-91
Number of pages9
JournalHistology and Histopathology
Volume7
Issue number1
Publication statusPublished - 1992

Fingerprint

Rana esculenta
Rana temporaria
Myenteric Plexus
Neurons
Duodenum
Silver
Intestines
Dihydrolipoamide Dehydrogenase
Enteric Nervous System
Acetylcholinesterase
Substance P
Nerve Fibers
Ganglia
Anura
gamma-Aminobutyric Acid
Small Intestine
Smooth Muscle Myocytes
Catecholamines
Vertebrates

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy
  • Histology
  • Pathology and Forensic Medicine

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Some morphological and histochemical features of the midgut myenteric plexus of the common European frog, Rana esculenta. / Gábriel, R.; Fekete, E.; Csoknya, M.

In: Histology and Histopathology, Vol. 7, No. 1, 1992, p. 83-91.

Research output: Contribution to journalArticle

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