Somatic embryogenesis in a broad spectrum of grape genotypes

Róbert Oláh, Anikó Zok, A. Pedryc, Susanne Howard, László G. Kovács

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Somatic embryogenesis is the preferred method for cell-to-plant regeneration of grapevine. In this study, we tested the embryogenic capacity of anther-derived calli from 59 grape genotypes, representing a diverse group of Vitis vinifera and hybrid varieties, and hybrids and accessions of non-vinifera Vitis species. Most genotypes were tested on two types of media: MST1 medium, which contained plant growth regulators (PGRs) 2,4-dichlorophenoxyacetic acid (2,4-D) and thidiazuron (TDZ), and MSE medium, which contained 2,4-D and 6-benzylaminopurine (BAP). Twenty-four of the grape genotypes produced embryogenic callus on one or both of these media, eighteen of which have not been reported to form somatic embryos before. The results also suggested that the various PGR combinations are differentially effective at inducing somatic embryos in various classes of grape genotypes. For example, seven of the eight V. vinifera conv. occidentalis varieties brought forth somatic embryos on MSE medium, and three out of four American Vitis genotypes produced somatic embryos on MST1 medium. We could not observe any apparent association between frequency of callus formation and embryogenic capacity of the anthers.

Original languageEnglish
Pages (from-to)134-137
Number of pages4
JournalScientia Horticulturae
Volume120
Issue number1
DOIs
Publication statusPublished - Mar 3 2009

Fingerprint

somatic embryogenesis
grapes
somatic embryos
genotype
2,4-D
Vitis
plant growth substances
Vitis vinifera
anthers
callus
callus formation
benzyladenine
cells
methodology

Keywords

  • Anther
  • Callus
  • Somatic embryogenesis
  • Thidiazuron
  • Vitis

ASJC Scopus subject areas

  • Horticulture

Cite this

Somatic embryogenesis in a broad spectrum of grape genotypes. / Oláh, Róbert; Zok, Anikó; Pedryc, A.; Howard, Susanne; Kovács, László G.

In: Scientia Horticulturae, Vol. 120, No. 1, 03.03.2009, p. 134-137.

Research output: Contribution to journalArticle

Oláh, Róbert ; Zok, Anikó ; Pedryc, A. ; Howard, Susanne ; Kovács, László G. / Somatic embryogenesis in a broad spectrum of grape genotypes. In: Scientia Horticulturae. 2009 ; Vol. 120, No. 1. pp. 134-137.
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