Somatic cell fusion of Trichoderma reesei resulting in new genetic combinations

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Abstract

A selection method has been developed for the isolation of recombinant strains of Trichoderma reesei QM 9414. The method is based on somatic hybridization via anastomosis or protoplast fusion, and on the difference in growth rate of the resulting heterokaryons and synkaryons. The more intensive growth of the synkaryons as due to allelic complementation of adenine-requiring auxotrophic strains mutated in the adenylosuccinate synthetase gene. The synkaryons appeared is energetically growing spots in the heterokaryotic background. Stable diploids could not be isolated, which points to the transient nature of the diploid state in this species.

Original languageEnglish
Pages (from-to)72-76
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume22
Issue number1
DOIs
Publication statusPublished - May 1985

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Adenylosuccinate Synthase
Trichoderma
Cell Fusion
Adenine
Diploidy
Fusion reactions
Genes
Protoplasts
Growth

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology
  • Bioengineering
  • Biotechnology

Cite this

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AB - A selection method has been developed for the isolation of recombinant strains of Trichoderma reesei QM 9414. The method is based on somatic hybridization via anastomosis or protoplast fusion, and on the difference in growth rate of the resulting heterokaryons and synkaryons. The more intensive growth of the synkaryons as due to allelic complementation of adenine-requiring auxotrophic strains mutated in the adenylosuccinate synthetase gene. The synkaryons appeared is energetically growing spots in the heterokaryotic background. Stable diploids could not be isolated, which points to the transient nature of the diploid state in this species.

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