Solvent-free enzymatic process for biolubricant production in continuous microfluidic reactor

J. Madarász, D. Németh, J. Bakos, L. Gubicza, P. Bakonyi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Abstract Synthesis of environmental-friendly chemicals attracts high attention nowadays, especially when naturally occurring compounds and industrial side-products are utilized. This paper reports for the first time on biolubricant (isoamyl oleate) production via continuous enzymatic reaction in microfluidic reactors (H-Cube™, X-Cube™). The proposed esterification was catalyzed using Novozym 435 enzyme, while substrates used were renewable raw materials: oleic acid and isoamyl alcohol. The latter is a main component of fusel oil, the by-product of bioethanol technology. The aim of this investigation was to evaluate the feasibility of the above-mentioned bioconversion in solvent-free media, considered as a cleaner way ahead. The successful applicability of miniaturized, continuous flow reactors for the biocatalytic reaction was demonstrated since isoamyl oleate could be obtained as long as 144 h in the H-Cube™ without any significant loss of enzyme activity. The highest conversion (98%) was achieved in X-Cube™. The results indicated that biocatalysts loading, residence time of reactants and dewatering of reaction mixture are important parameters and facilitate improved process efficiency.

Original languageEnglish
Article number5105
Pages (from-to)140-144
Number of pages5
JournalJournal of Cleaner Production
Volume93
DOIs
Publication statusPublished - Apr 15 2015

Fingerprint

Bioconversion
Bioethanol
Biocatalysts
Oleic acid
Dewatering
Esterification
Enzyme activity
Microfluidics
Byproducts
Raw materials
Alcohols
Enzymes
Substrates
dewatering
enzyme activity
alcohol
residence time
enzyme
substrate
oil

Keywords

  • Biolubricant
  • Enzymatic synthesis
  • Isoamyl oleate
  • Microfluidic reactor
  • Novozym 435

ASJC Scopus subject areas

  • Industrial and Manufacturing Engineering
  • Renewable Energy, Sustainability and the Environment
  • Environmental Science(all)
  • Strategy and Management

Cite this

Solvent-free enzymatic process for biolubricant production in continuous microfluidic reactor. / Madarász, J.; Németh, D.; Bakos, J.; Gubicza, L.; Bakonyi, P.

In: Journal of Cleaner Production, Vol. 93, 5105, 15.04.2015, p. 140-144.

Research output: Contribution to journalArticle

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