Solid-state fermentation for the production of biomass valorizing feruloyl esterase

Nishant Gopalan, Kesavan Madhavan Nampoothiri, G. Szakács, Binod Parameswaran, Ashok Pandey

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aspergillus niger ATCC 13497 has been used to produce feruloyl esterase type A enzyme. The process parameters were optimized to attain a maximum of 11984.1 U/gds of enzyme activity within a period of 72 h using destarched wheat bran as a substrate. The purified enzyme studied in the present work displays a pH optimum of 5, and a temperature optimum of 37 °C. The enzyme has a size of 35 kDa and exhibited moderate thermostability, with 41% of activity retained at 90 °C. Ni +2 and Co+2 ions were found to have stimulatory activity on the enzyme with 66% and 78% increase in activity respectively. Cu+2 inhibited the activity by 88%. Statistical optimization enhanced the enzyme activity by 2.47 fold.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalBiocatalysis and Agricultural Biotechnology
Volume7
DOIs
Publication statusPublished - Jul 1 2016

Keywords

  • AnFaeA
  • Aspergillus niger
  • Bioprocess
  • Feruloyl esterase
  • Solid state fermentation (SSF)

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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