Solid-state fermentation for production of phytase by Rhizopus oligosporus

A. Sabu, S. Sarita, A. Pandey, B. Bogar, G. Szakács, C. R. Soccol

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Solid-state fermentation of coconut oil cake has been carried out with Rhizopus oligosporus for the production of phytase. Phytase is used commercially in the animal feed industry to improve animal performance because there is a substantial and growing interest among swine and poultry producers in the application of phytase to improve the nutritional quality in animal feeds. Demonstrated benefits include improved feed yield ratios and reduction in the environmental costs associated with the disposal of animal wastes. We report the production of extracellular phytase by R. oligosporus under solid-state fermentation using coconut oil cake as substrate. Maximal enzyme production (14.29 U/g of dry substrate) occurred at pH 5.3, 30°C, and 54.5% moisture content after 96 h of incubation. The addition of extra nutrients to the substrate resulted in inhibition of product formation. The results indicate the scope for production of phytase using coconut oil cake as solid substrate without additional nutrients.

Original languageEnglish
Pages (from-to)251-260
Number of pages10
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume102-103
DOIs
Publication statusPublished - Jul 2002

Fingerprint

6-Phytase
Rhizopus
Fermentation
Animals
Substrates
Nutrients
Poultry
Food
Waste disposal
Nutritive Value
Moisture
Enzymes
Industry
Swine
Costs and Cost Analysis
Oils
Costs
coconut oil

Keywords

  • Coconut oil cake
  • Phytase
  • Rhizopus oligosporus
  • Solid-state fermentation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

Solid-state fermentation for production of phytase by Rhizopus oligosporus. / Sabu, A.; Sarita, S.; Pandey, A.; Bogar, B.; Szakács, G.; Soccol, C. R.

In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 102-103, 07.2002, p. 251-260.

Research output: Contribution to journalArticle

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