Solid-state and solution conformation of 3′-amino-3′-deoxythymidine, precursor to a noncompetitive inhibitor of HIV-1 reverse transcriptase

Teréz Kovács, László Párkányi, L. Párkányí, Francisco Cervantes-Lee, Keith H. Pannell, Paul F. Torrence

Research output: Contribution to journalArticle

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Abstract

The recent finding that 3′-amino-3′-deoxythyinidine 5′-triphosphate is a noncompetitive inhibitor of the HIV-1 reverse transcriptase (Kedar, P. S.; et al. Biochemistry 1990, 29, 3603-3611), prompted an investigation of the conformation of 3′-amino-3′-deoxythymidine. An X-ray diffraction study has revealed that the glycosidic torsion angle of the nucleoside is in the less common syn region and this solid-state geometry is stabilized by a three-dimensional network of self-associated hydrogen-bonded molecules. On the other hand, the aqueous solution conformation, as determined by 1H NMR, places the glycosidic torsion angle in the more usual anti region with the sugar in an equilibrium between C3′-endo and C2′-endo puckering. The energy barrier between the solid-state and solution conformation is relatively low as was demonstrated by the MM2 calculations.

Original languageEnglish
Pages (from-to)2595-2600
Number of pages6
JournalJournal of Medicinal Chemistry
Volume34
Issue number8
Publication statusPublished - 1991

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Conformations
Torsional stress
Nucleosides
X-Ray Diffraction
Biochemistry
Hydrogen
Energy barriers
Sugars
Nuclear magnetic resonance
X ray diffraction
Molecules
Geometry
Human immunodeficiency virus 1 reverse transcriptase
3'-aminothymidine
Proton Magnetic Resonance Spectroscopy
triphosphoric acid

ASJC Scopus subject areas

  • Organic Chemistry

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Solid-state and solution conformation of 3′-amino-3′-deoxythymidine, precursor to a noncompetitive inhibitor of HIV-1 reverse transcriptase. / Kovács, Teréz; Párkányi, László; Párkányí, L.; Cervantes-Lee, Francisco; Pannell, Keith H.; Torrence, Paul F.

In: Journal of Medicinal Chemistry, Vol. 34, No. 8, 1991, p. 2595-2600.

Research output: Contribution to journalArticle

Kovács, Teréz ; Párkányi, László ; Párkányí, L. ; Cervantes-Lee, Francisco ; Pannell, Keith H. ; Torrence, Paul F. / Solid-state and solution conformation of 3′-amino-3′-deoxythymidine, precursor to a noncompetitive inhibitor of HIV-1 reverse transcriptase. In: Journal of Medicinal Chemistry. 1991 ; Vol. 34, No. 8. pp. 2595-2600.
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AU - Cervantes-Lee, Francisco

AU - Pannell, Keith H.

AU - Torrence, Paul F.

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