Solid-phase microextraction: A powerful sample preparation tool prior to mass spectrometric analysis

György Vas, K. Vékey

Research output: Contribution to journalArticle

379 Citations (Scopus)

Abstract

Sample preparation is an essential step in analysis, greatly influencing the reliability and accuracy of resulted the time and cost of analysis. Solid-Phase Microextraction (SPME) is a very simple and efficient, solventless sample preparation method, invented by Pawliszyn in 1989. SPME has been widely used in different fields of analytical chemistry since its first applications to environmental and food analysis and is ideally suited for coupling with mass spectrometry (MS). All steps of the conventional liquid-liquid extraction (LLE) such as extraction, concentration, (derivatization) and transfer to the chromatograph are integrated into one step and one device, considerably simplifying the sample preparation procedure. It uses a fused-silica fibre that is coated on the outside with an appropriate stationary phase. The analytes in the sample are directly extracted to the fibre coating. The SPME technique can be routinely used in combination with gas chromatography, high-performance liquid chromatography and capillary electrophoresis and places no restriction on MS. SPME reduces the time necessary for sample preparation, decreases purchase and disposal costs of solvents and can improve detection limits. The SPME technique is ideally suited for MS applications, combining a simple and efficient sample preparation with versatile and sensitive detection. This review summarizes analytical characteristics and variants of the SPME technique and its applications in combination with MS.

Original languageEnglish
Pages (from-to)233-254
Number of pages22
JournalJournal of Mass Spectrometry
Volume39
Issue number3
DOIs
Publication statusPublished - Mar 2004

Fingerprint

Solid Phase Microextraction
solid phases
preparation
Mass spectrometry
Mass Spectrometry
mass spectroscopy
analytical chemistry
Food Analysis
costs
Costs and Cost Analysis
Capillary electrophoresis
Liquid-Liquid Extraction
fibers
Fibers
disposal
liquid chromatography
Capillary Electrophoresis
High performance liquid chromatography
Liquids
Fused silica

Keywords

  • Capillary electrophoresis/mass spectrometry
  • Environmental chemisty
  • Food analysis
  • Forensic analysis
  • Gas chromatography
  • Gas chromatography/mass spectrometry
  • Headspace
  • High-performance liquid chromatography
  • High-performance liquid chromatography/mass spectrometry
  • Pharmaceuticals
  • Pharmakokinetics
  • Solid-phase microextraction
  • Solid-phase microextraction/matrix-assisted laser desorption/ionization mass spectrometry
  • Wine

ASJC Scopus subject areas

  • Organic Chemistry
  • Spectroscopy
  • Biophysics

Cite this

Solid-phase microextraction : A powerful sample preparation tool prior to mass spectrometric analysis. / Vas, György; Vékey, K.

In: Journal of Mass Spectrometry, Vol. 39, No. 3, 03.2004, p. 233-254.

Research output: Contribution to journalArticle

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