Softening of the packing density of horseradish peroxidase by a H-donor bound near the heme pocket

J. Fidy, J. M. Vanderkooi, J. Zollfrank, J. Friedrich

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We use pressure tuning of spectral holes to estimate the compressibility of protein molecules by optical means. We found that the compressibility of mesoporphyrin-substituted horseradish peroxidase increases by a factor of three when it incorporates small aromatic H-donor molecules that bind in the vicinity of its heme pocket. Such a dramatic softening of its packing density corresponds to a jump from a compressibility range characteristic for the solid state into that characteristic for liquids.

Original languageEnglish
Pages (from-to)1605-1612
Number of pages8
JournalBiophysical Journal
Volume63
Issue number6
Publication statusPublished - 1992

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Horseradish Peroxidase
Heme
Pressure
Proteins
mesoporphyrin IX

ASJC Scopus subject areas

  • Biophysics

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Softening of the packing density of horseradish peroxidase by a H-donor bound near the heme pocket. / Fidy, J.; Vanderkooi, J. M.; Zollfrank, J.; Friedrich, J.

In: Biophysical Journal, Vol. 63, No. 6, 1992, p. 1605-1612.

Research output: Contribution to journalArticle

Fidy, J, Vanderkooi, JM, Zollfrank, J & Friedrich, J 1992, 'Softening of the packing density of horseradish peroxidase by a H-donor bound near the heme pocket', Biophysical Journal, vol. 63, no. 6, pp. 1605-1612.
Fidy, J. ; Vanderkooi, J. M. ; Zollfrank, J. ; Friedrich, J. / Softening of the packing density of horseradish peroxidase by a H-donor bound near the heme pocket. In: Biophysical Journal. 1992 ; Vol. 63, No. 6. pp. 1605-1612.
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