Sodium salicylate inhibits NF-κB and induces apoptosis in PC12 cells

K. Kiss, J. Kiss, E. Rudolf, M. Červinka, J. Szeberényi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Sodium salicylate (NaSal) is an effective analgetic and antiinflammatory drug. Beside its well-known inhibitory effect on the cyclooxigenase enzymes, it influences the activity of other signal transduction proteins including nuclear factor kappa B (NF-κB) transcription factor. NF-κB is found in the cytoplasm bound to an inhibitory protein, inhibitory kappa B (IκB). After its phosphorylation, IκB is degraded and the released NF-κB translocates into the nucleus. Sodium salicylate inhibits the degradation of IκB, thus, NF-κB activation cannot occur. According to previous observations, the inhibition of this activation can lead to apoptosis. The main goals of this study were to demonstrate that inhibition of NF-κB by sodium salicylate decreases the viability of rat phaeochromocytoma PC12 cells and to investigate the nature of cell damage and death. PC12 cells were treated with different concentrations of sodium salicylate (1-20 mM). Higher concentrations (10-20 mM) killed PC12 cells in a dose-dependent manner. The assessments were done by direct cell counting in a Bürker chamber and by the WST-1 cytotoxicity assay. We also found a decreased NF-κB activity after sodium salicylate treatment by electrophoretic mobility shift assay (EMSA). The cells treated with sodium salicylate were undergoing apoptosis as seen on our records obtained by time-lapse videomicroscopy as well as shown by DNA fragmentation experiments. The decreased DNA binding activity of NF-κB indicates that the inhibition of NF-κB can play a role in these processes.

Original languageEnglish
Pages (from-to)229-240
Number of pages12
JournalJournal of Biochemical and Biophysical Methods
Volume61
Issue number1-2 SPEC. ISS.
DOIs
Publication statusPublished - Oct 29 2004

Fingerprint

Sodium Salicylate
NF-kappa B
PC12 Cells
Apoptosis
Assays
Chemical activation
Video Microscopy
Electrophoretic mobility
Signal transduction
Phosphorylation
DNA
Pheochromocytoma
Electrophoretic Mobility Shift Assay
DNA Fragmentation
Cytotoxicity
Nose
Rats
Signal Transduction
Cytoplasm
Proteins

Keywords

  • Apoptosis
  • NF-κB
  • NGF
  • PC12 cells
  • Sodium salicylate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Sodium salicylate inhibits NF-κB and induces apoptosis in PC12 cells. / Kiss, K.; Kiss, J.; Rudolf, E.; Červinka, M.; Szeberényi, J.

In: Journal of Biochemical and Biophysical Methods, Vol. 61, No. 1-2 SPEC. ISS., 29.10.2004, p. 229-240.

Research output: Contribution to journalArticle

Kiss, K. ; Kiss, J. ; Rudolf, E. ; Červinka, M. ; Szeberényi, J. / Sodium salicylate inhibits NF-κB and induces apoptosis in PC12 cells. In: Journal of Biochemical and Biophysical Methods. 2004 ; Vol. 61, No. 1-2 SPEC. ISS. pp. 229-240.
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