Sodium butyrate modifies the stabilizing complexes of tyrosine hydroxylase mRNA

T. Arányi, C. Sarkis, S. Berrard, K. Sardin, V. Siron, O. Khalfallah, J. Mallet

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Multiple mechanisms regulate the expression of the tyrosine hydroxylase (Th) gene, which encodes the rate-limiting enzyme in the biosynthesis of catecholamines. Sodium butyrate (SOB), a physiological histone deacetylase (HDAC) inhibitor, was reported to stimulate the Th gene promoter activity in reporter gene assays. However, the expression of the endogenous Th gene in PC12 cells was reported to be either stimulated or inhibited by SOB. Here, we report that SOB and other HDAC inhibitors drastically (up to 90%) and reversibly decrease the level of TH mRNA in PC12 cells. We also show that SOB does not influence the transcription initiation rate of the Th gene but perturbs the formation of protein-RNA complexes at the 3′UTR of the gene. Our results suggest that SOB inhibits the expression of the Th gene by destabilizing TH mRNAs.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume359
Issue number1
DOIs
Publication statusPublished - Jul 20 2007

Fingerprint

Butyric Acid
Tyrosine 3-Monooxygenase
Genes
Messenger RNA
Histone Deacetylase Inhibitors
PC12 Cells
Reporter Genes
Biosynthesis
Transcription
Catecholamines
Assays
RNA
Enzymes
Proteins

Keywords

  • HDAC
  • Histone acetylation
  • PC12
  • RNA stability
  • Run-on assay
  • Sodium butyrate
  • TSA
  • Tyrosine hydroxylase
  • UV crosslinking

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sodium butyrate modifies the stabilizing complexes of tyrosine hydroxylase mRNA. / Arányi, T.; Sarkis, C.; Berrard, S.; Sardin, K.; Siron, V.; Khalfallah, O.; Mallet, J.

In: Biochemical and Biophysical Research Communications, Vol. 359, No. 1, 20.07.2007, p. 15-19.

Research output: Contribution to journalArticle

Arányi, T. ; Sarkis, C. ; Berrard, S. ; Sardin, K. ; Siron, V. ; Khalfallah, O. ; Mallet, J. / Sodium butyrate modifies the stabilizing complexes of tyrosine hydroxylase mRNA. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 359, No. 1. pp. 15-19.
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