SNOM Imaging of a Crypt-Like Feature in Adenocarcinoma Associated with Barrett's Oesophagus

Timothy Craig, Andrew D. Smith, Gareth M. Holder, James Ingham, Caroline I. Smith, A. Varró, D. Mark Pritchard, Steve D. Barrett, David S. Martin, Paul Harrison, Andrzej Wolski, Antonio Cricenti, Marco Luce, Mark Surman, Swapan Chattopadhyay, Peter Weightman, Michele R.F. Siggel-King

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The development of more accurate and sensitive diagnostic techniques is a key factor in efforts to improve cancer survival rates. The technique of infrared aperture fibre scanning near-field optical microscopy (IR-SNOM), together with radiation from the infrared free-electron laser (IR-FEL) on ALICE at Daresbury Laboratory (UK), has been used to obtain IR images of a crypt-like feature and the surrounding tissue; the tissue was taken from a patient with oesophageal adenocarcinoma and with a history of Barrett's oesophagus. We have shown that the DNA signal is enhanced relative to other contributions in the region of the crypt, and the glycoprotein signal shows a less pronounced increase in the region of the crypt. The Amide II signal is found to be anti-correlated with the DNA and glycoprotein profiles. The absorbance of the Amide II signal is found to differ for three different types of cancer tissue. High-resolution IR images of the crypt reveal additional structure that would not be resolved in diffraction-limited techniques.

Original languageEnglish
Article number1700518
JournalPhysica Status Solidi (B) Basic Research
Volume255
Issue number4
DOIs
Publication statusPublished - Apr 1 2018

Fingerprint

Near field scanning optical microscopy
esophagus
Glycoproteins
Tissue
Amides
Imaging techniques
DNA
amides
deoxyribonucleic acid
Infrared lasers
cancer
Free electron lasers
Diffraction
free electron lasers
infrared lasers
Infrared radiation
Radiation
near fields
Fibers
apertures

Keywords

  • adenocarcinoma
  • DNA
  • infrared radiation
  • scanning near-field optical microscopy
  • tissue

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Craig, T., Smith, A. D., Holder, G. M., Ingham, J., Smith, C. I., Varró, A., ... Siggel-King, M. R. F. (2018). SNOM Imaging of a Crypt-Like Feature in Adenocarcinoma Associated with Barrett's Oesophagus. Physica Status Solidi (B) Basic Research, 255(4), [1700518]. https://doi.org/10.1002/pssb.201700518

SNOM Imaging of a Crypt-Like Feature in Adenocarcinoma Associated with Barrett's Oesophagus. / Craig, Timothy; Smith, Andrew D.; Holder, Gareth M.; Ingham, James; Smith, Caroline I.; Varró, A.; Pritchard, D. Mark; Barrett, Steve D.; Martin, David S.; Harrison, Paul; Wolski, Andrzej; Cricenti, Antonio; Luce, Marco; Surman, Mark; Chattopadhyay, Swapan; Weightman, Peter; Siggel-King, Michele R.F.

In: Physica Status Solidi (B) Basic Research, Vol. 255, No. 4, 1700518, 01.04.2018.

Research output: Contribution to journalArticle

Craig, T, Smith, AD, Holder, GM, Ingham, J, Smith, CI, Varró, A, Pritchard, DM, Barrett, SD, Martin, DS, Harrison, P, Wolski, A, Cricenti, A, Luce, M, Surman, M, Chattopadhyay, S, Weightman, P & Siggel-King, MRF 2018, 'SNOM Imaging of a Crypt-Like Feature in Adenocarcinoma Associated with Barrett's Oesophagus', Physica Status Solidi (B) Basic Research, vol. 255, no. 4, 1700518. https://doi.org/10.1002/pssb.201700518
Craig, Timothy ; Smith, Andrew D. ; Holder, Gareth M. ; Ingham, James ; Smith, Caroline I. ; Varró, A. ; Pritchard, D. Mark ; Barrett, Steve D. ; Martin, David S. ; Harrison, Paul ; Wolski, Andrzej ; Cricenti, Antonio ; Luce, Marco ; Surman, Mark ; Chattopadhyay, Swapan ; Weightman, Peter ; Siggel-King, Michele R.F. / SNOM Imaging of a Crypt-Like Feature in Adenocarcinoma Associated with Barrett's Oesophagus. In: Physica Status Solidi (B) Basic Research. 2018 ; Vol. 255, No. 4.
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