Smad signal and TGFβ induced apoptosis in human lymphoma cells

A. Sebestyén, G. Barna, Katalin Nagy, Judit Jánosi, S. Paku, Eszter Kohut, L. Berczi, R. Mihalik, L. Kópper

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Transforming growth factor β1 (TGFβ1) has antiproliferative and/or apoptotic effect on lymphoid cells. In certain lymphomas exogenous TGFβ1 is able to induce apoptosis, however many lymphoid malignancies are resistant to the endogenous TGFβ1 production. We studied the expression and the activity of TGFβ1 signalling components in B cell lymphoma cell lines (e.g. HT58 cells) and in isolated human peripheral mononuclear cells (PBMCs) from healthy individual's and B-CLL patient's blood. We found that all signal transducer Smads (Smad2,-3; Smad4) and at least one of the inhibitory Smads (Smad6,-7) were expressed in non-treated lymphoma cells, but the inhibitory Smads did not in normal/control PBMCs. However, after TGFβ1 treatment Smad6 disappeared, while the expression of Smad7 increased in HT58 cells. The activity of Smad signals was proved by phosphorylation of Smad2, nuclear translocation of Smad2/3, and the increased expression of Smad-dependent gene, TIEG in TGFβ1 treated lymphoma cells. These results showed that Smad signaling is available in certain different human lymphoma cells, however ISmads expression could inhibit the signal transmission. This findings indicates that the lost sensitivity of lymphoma cells toward a physiological regulatory factor could be reversed.

Original languageEnglish
Pages (from-to)228-235
Number of pages8
JournalCytokine
Volume30
Issue number5
DOIs
Publication statusPublished - Jun 7 2005

Fingerprint

Transforming Growth Factors
Lymphoma
Apoptosis
Cells
Phosphorylation
B-Cell Lymphoma
Transducers
Blood
Genes
Lymphocytes
Cell Line
Neoplasms

Keywords

  • Apoptosis
  • Lymphoma
  • Smad-signal
  • TGFβ

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Smad signal and TGFβ induced apoptosis in human lymphoma cells. / Sebestyén, A.; Barna, G.; Nagy, Katalin; Jánosi, Judit; Paku, S.; Kohut, Eszter; Berczi, L.; Mihalik, R.; Kópper, L.

In: Cytokine, Vol. 30, No. 5, 07.06.2005, p. 228-235.

Research output: Contribution to journalArticle

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