Slow regeneration of inmune complex binding sites on the macrophage surface after previous digestion with pronase

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Abstract

Peroxidase-antiperoxidase (PAP) binding sites on the rat peritoneal macrophage surface were damaged by pronase digestion and the reappearance of functionally intact receptors was investigated morphologically and spectrophotometrically. Pronase digestion decreased the PAP binding ability of macrophages to about 40% of the original value. Removing the pronase the regeneration of ligand binding was very protracted with only about 60% intact receptors even after 30 min. From these findings we conclude that the recycling of internalized Fc receptors greatly contribute to the replenishment of receptors on the cell surface.

Original languageEnglish
Pages (from-to)289-298
Number of pages10
JournalCell Biology International Reports
Volume12
Issue number4
DOIs
Publication statusPublished - 1988

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Pronase
Regeneration
Digestion
Macrophages
Binding Sites
Peroxidase
Fc Receptors
Peritoneal Macrophages
Recycling
Cell Surface Receptors
Ligands

ASJC Scopus subject areas

  • Cell Biology

Cite this

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abstract = "Peroxidase-antiperoxidase (PAP) binding sites on the rat peritoneal macrophage surface were damaged by pronase digestion and the reappearance of functionally intact receptors was investigated morphologically and spectrophotometrically. Pronase digestion decreased the PAP binding ability of macrophages to about 40{\%} of the original value. Removing the pronase the regeneration of ligand binding was very protracted with only about 60{\%} intact receptors even after 30 min. From these findings we conclude that the recycling of internalized Fc receptors greatly contribute to the replenishment of receptors on the cell surface.",
author = "A. Kiss and P. R{\"o}hlich",
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AB - Peroxidase-antiperoxidase (PAP) binding sites on the rat peritoneal macrophage surface were damaged by pronase digestion and the reappearance of functionally intact receptors was investigated morphologically and spectrophotometrically. Pronase digestion decreased the PAP binding ability of macrophages to about 40% of the original value. Removing the pronase the regeneration of ligand binding was very protracted with only about 60% intact receptors even after 30 min. From these findings we conclude that the recycling of internalized Fc receptors greatly contribute to the replenishment of receptors on the cell surface.

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