Sleeping Beauty transposon-based system for cellular reprogramming and targeted gene insertion in induced pluripotent stem cells

Ivana Grabundzija, Jichang Wang, Attila Sebe, Zsuzsanna Erdei, Robert Kajdi, Anantharam Devaraj, Doris Steinemann, Károly Szuhai, Ulrike Stein, Tobias Cantz, Axel Schambach, Christopher Baum, Zsuzsanna Izsvák, Balázs Sarkadi, Zoltán Ivics

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Abstract

The discovery of direct cell reprogramming and induced pluripotent stem (iPS) cell technology opened up new avenues for the application of nonviral, transposon-based gene delivery systems. The Sleeping Beauty (SB) transposon is highly advanced for versatile genetic manipulations in mammalian cells. We established iPS cell reprogramming of mouse embryonic fibroblasts and human foreskin fibroblasts by transposition of OSKM (Oct4, Sox2, Klf4 and c-Myc) and OSKML (OSKM+Lin28) expression cassettes mobilized by the SB100X hyperactive transposase. The efficiency of iPS cell derivation with SB transposon system was in the range of that obtained with retroviral vectors. Co-expression of the miRNA302/367 cluster together with OSKM significantly improved reprogramming efficiency and accelerated the temporal kinetics of reprogramming. The iPS cells displayed a stable karyotype, and hallmarks of pluripotency including expression of stem cell markers and the ability to differentiate into embryoid bodies in vitro. We demonstrate Cre recombinasemediated exchange allowing simultaneous removal of the reprogramming cassette and targeted knock-in of an expression cassette of interest into the transposon-tagged locus in mouse iPS cells. This strategy would allow correction of a genetic defect by site-specific insertion of a therapeutic gene construct into 'safe harbor' sites in the genomes of autologous, patient-derived iPS cells.

Original languageEnglish
Pages (from-to)1829-1847
Number of pages19
JournalNucleic acids research
Volume41
Issue number3
DOIs
Publication statusPublished - Feb 1 2013

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ASJC Scopus subject areas

  • Genetics

Cite this

Grabundzija, I., Wang, J., Sebe, A., Erdei, Z., Kajdi, R., Devaraj, A., Steinemann, D., Szuhai, K., Stein, U., Cantz, T., Schambach, A., Baum, C., Izsvák, Z., Sarkadi, B., & Ivics, Z. (2013). Sleeping Beauty transposon-based system for cellular reprogramming and targeted gene insertion in induced pluripotent stem cells. Nucleic acids research, 41(3), 1829-1847. https://doi.org/10.1093/nar/gks1305