Sleep loss alters hypothalamic growth hormone-releasing hormone receptors in rats

J. Gardi, P. Taishi, R. Speth, F. Obal, J. M. Krueger

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10 Citations (Scopus)

Abstract

Previous experiments suggest that sleep deprivation (SD) is associated with growth hormone-releasing hormone (GHRH) release and that GHRH promotes sleep via intrahypothalamic sites of action. Binding of [His1, 125I-Tyr10, Nle27]hGHRH(1-32) amide and GHRH receptor (GHRH-R) mRNA levels were determined in the hypothalamus and pituitary of rats subjected to 8 h of SD and of undisturbed control rats. The characteristics of the hypothalamic GHRH binding sites differed from those of the pituitary. High affinity GHRH binding and GHRH-R mRNA levels decreased by 50% in the hypothalamus of SD rats, whereas there were no alterations in the pituitary. The results demonstrate that GHRH-Rs exist in the hypothalamus and they respond differently to SD than the GHRH-Rs in the pituitary. The SD-induced changes are explained by down-regulation of the hypothalamic GHRH-Rs induced by GHRH release during and after SD.

Original languageEnglish
Pages (from-to)69-72
Number of pages4
JournalNeuroscience Letters
Volume329
Issue number1
DOIs
Publication statusPublished - Aug 23 2002

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Keywords

  • Brain
  • Growth hormone-releasing hormone receptor
  • Messenger RNA
  • Pituitary
  • Sleep
  • Somatotropic axis

ASJC Scopus subject areas

  • Neuroscience(all)

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