Sleep loss alters hypothalamic growth hormone-releasing hormone receptors in rats

J. Gardi, P. Taishi, R. Speth, F. Obal, J. M. Krueger

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Previous experiments suggest that sleep deprivation (SD) is associated with growth hormone-releasing hormone (GHRH) release and that GHRH promotes sleep via intrahypothalamic sites of action. Binding of [His1, 125I-Tyr10, Nle27]hGHRH(1-32) amide and GHRH receptor (GHRH-R) mRNA levels were determined in the hypothalamus and pituitary of rats subjected to 8 h of SD and of undisturbed control rats. The characteristics of the hypothalamic GHRH binding sites differed from those of the pituitary. High affinity GHRH binding and GHRH-R mRNA levels decreased by 50% in the hypothalamus of SD rats, whereas there were no alterations in the pituitary. The results demonstrate that GHRH-Rs exist in the hypothalamus and they respond differently to SD than the GHRH-Rs in the pituitary. The SD-induced changes are explained by down-regulation of the hypothalamic GHRH-Rs induced by GHRH release during and after SD.

Original languageEnglish
Pages (from-to)69-72
Number of pages4
JournalNeuroscience Letters
Volume329
Issue number1
DOIs
Publication statusPublished - Aug 23 2002

Fingerprint

Hypothalamic Hormones
Growth Hormone-Releasing Hormone
Sleep
Sleep Deprivation
Hypothalamus
somatotropin releasing hormone receptor
Messenger RNA
Amides
Down-Regulation
Binding Sites

Keywords

  • Brain
  • Growth hormone-releasing hormone receptor
  • Messenger RNA
  • Pituitary
  • Sleep
  • Somatotropic axis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sleep loss alters hypothalamic growth hormone-releasing hormone receptors in rats. / Gardi, J.; Taishi, P.; Speth, R.; Obal, F.; Krueger, J. M.

In: Neuroscience Letters, Vol. 329, No. 1, 23.08.2002, p. 69-72.

Research output: Contribution to journalArticle

Gardi, J. ; Taishi, P. ; Speth, R. ; Obal, F. ; Krueger, J. M. / Sleep loss alters hypothalamic growth hormone-releasing hormone receptors in rats. In: Neuroscience Letters. 2002 ; Vol. 329, No. 1. pp. 69-72.
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