Skeletal muscles, heart, and lung are the main sources of oxygen radicals in old rats

Andrey V. Kozlov, L. Szalay, Fraz Umar, Karl Kropik, Katrin Staniek, Hans Niedermüller, Soheyl Bahrami, Hans Nohl

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The aim of this study was to compare rat tissues with respect to their reactive oxygen and nitrogen species (RONS) generating activities as a function of age. We quantified the RONS generation in vivo in young (6 months) and in old (30 months) male Sprague-Dawley rats using the recently developed spin trap 1-hydroxy-3-carboxy-pyrrolidine, applied intravenously. This spin trap reacts with superoxide radical and peroxynitrite yielding a stable spin adduct which is detectable by means of electron paramagnetic resonance (EPR) spectroscopy in frozen tissues. In old rats RONS generation was significantly increased compared to their young counterparts in the following order: blood

Original languageEnglish
Pages (from-to)382-389
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1740
Issue number3
DOIs
Publication statusPublished - Jun 10 2005

Fingerprint

Reactive Nitrogen Species
Reactive Oxygen Species
Skeletal Muscle
Lung
Peroxynitrous Acid
Electron Spin Resonance Spectroscopy
Superoxides
Sprague Dawley Rats
Spectrum Analysis

Keywords

  • Aging
  • Electron paramagnetic resonance
  • Mitochondria
  • Rat
  • Reactive oxygen and nitrogen species
  • Spin trapping

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Biophysics

Cite this

Skeletal muscles, heart, and lung are the main sources of oxygen radicals in old rats. / Kozlov, Andrey V.; Szalay, L.; Umar, Fraz; Kropik, Karl; Staniek, Katrin; Niedermüller, Hans; Bahrami, Soheyl; Nohl, Hans.

In: Biochimica et Biophysica Acta - Molecular Basis of Disease, Vol. 1740, No. 3, 10.06.2005, p. 382-389.

Research output: Contribution to journalArticle

Kozlov, Andrey V. ; Szalay, L. ; Umar, Fraz ; Kropik, Karl ; Staniek, Katrin ; Niedermüller, Hans ; Bahrami, Soheyl ; Nohl, Hans. / Skeletal muscles, heart, and lung are the main sources of oxygen radicals in old rats. In: Biochimica et Biophysica Acta - Molecular Basis of Disease. 2005 ; Vol. 1740, No. 3. pp. 382-389.
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