Skeletal muscle intermediate filaments form a stress-transmitting and stress-signaling network

Michelle G. Palmisano, Shannon N. Bremner, Troy A. Hornberger, Gretchen A. Meyer, Andrea A. Domenighetti, Sameer B. Shah, Balázs Kiss, Miklos Kellermayer, Allen F. Ryan, Richard L. Lieber

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26 Citations (Scopus)

Abstract

A fundamental requirement of cells is their ability to transduce and interpret their mechanical environment. This ability contributes to regulation of growth, differentiation and adaptation in many cell types. The intermediate filament (IF) system not only provides passive structural support to the cell, but recent evidence points to IF involvement in active biological processes such as signaling, mechanotransduction and gene regulation. However, the mechanisms that underlie these processes are not well known. Skeletal muscle cells provide a convenient system to understand IF function because the major muscle-specific IF, desmin, is expressed in high abundance and is highly organized. Here, we show that desmin plays both structural and regulatory roles in muscle cells by demonstrating that desmin is required for the maintenance of myofibrillar alignment, nuclear deformation, stress production and JNK-mediated stress sensing. Finite element modeling of the muscle IF system suggests that desmin immediately below the sarcolemma is the most functionally significant. This demonstration of biomechanical integration by the desmin IF system suggests that it plays an active biological role in muscle in addition to its accepted structural role.

Original languageEnglish
Pages (from-to)219-224
Number of pages6
JournalJournal of cell science
Volume128
Issue number2
DOIs
Publication statusPublished - Jan 1 2015

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Keywords

  • Biomechanics
  • Desmin
  • Mechanotransduction
  • Tensegrity

ASJC Scopus subject areas

  • Cell Biology

Cite this

Palmisano, M. G., Bremner, S. N., Hornberger, T. A., Meyer, G. A., Domenighetti, A. A., Shah, S. B., Kiss, B., Kellermayer, M., Ryan, A. F., & Lieber, R. L. (2015). Skeletal muscle intermediate filaments form a stress-transmitting and stress-signaling network. Journal of cell science, 128(2), 219-224. https://doi.org/10.1242/jcs.142463