Size Selective Recognition of siRNA by an RNA Silencing Suppressor

Jeffrey M. Vargason, G. Szittya, J. Burgyán, Traci M. Tanaka Hall

Research output: Contribution to journalArticle

378 Citations (Scopus)

Abstract

RNA silencing in plants likely exists as a defense mechanism against molecular parasites such as RNA viruses, retrotransposons, and transgenes. As a result, many plant viruses have adapted mechanisms to evade and suppress gene silencing. Tombusviruses express a 19 kDa protein (p19), which has been shown to suppress RNA silencing in vivo and bind silencing-generated and synthetic small interfering RNAs (siRNAs) in vitro. Here we report the 2.5 Å crystal structure of p19 from the Carnation Italian ringspot virus (CIRV) bound to a 21 nt siRNA and demonstrate in biochemical and in vivo assays that CIRV p19 protein acts as a molecular caliper to specifically select siRNAs based on the length of the duplex region of the RNA.

Original languageEnglish
Pages (from-to)799-811
Number of pages13
JournalCell
Volume115
Issue number7
DOIs
Publication statusPublished - Dec 26 2003

Fingerprint

RNA Interference
Dianthus
Viruses
Small Interfering RNA
RNA
Tombusvirus
Plant Viruses
Retroelements
RNA Viruses
Gene Silencing
Transgenes
Parasites
Proteins
Assays
Genes
Crystal structure

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Size Selective Recognition of siRNA by an RNA Silencing Suppressor. / Vargason, Jeffrey M.; Szittya, G.; Burgyán, J.; Tanaka Hall, Traci M.

In: Cell, Vol. 115, No. 7, 26.12.2003, p. 799-811.

Research output: Contribution to journalArticle

Vargason, Jeffrey M. ; Szittya, G. ; Burgyán, J. ; Tanaka Hall, Traci M. / Size Selective Recognition of siRNA by an RNA Silencing Suppressor. In: Cell. 2003 ; Vol. 115, No. 7. pp. 799-811.
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