Singlet energy dissipation in the photosystem II light-harvesting complex does not involve energy transfer to carotenoids

Marc G. Müller, P. Lambrev, Michael Reus, Emilie Wientjes, Roberta Croce, Alfred R. Holzwarth

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

The energy dissipation mechanism in oligomers of the major light-harvesting complex II (LHC II) from Arabidopsis thaliana mutants npq1 and npq2, zeaxanthin-deficient and zeaxanthinnriched, respectively, has been studied by femtosecond tran-sient absorption. The kinetics obtained at different excitation intensities are compared and the implications of singlet-singlet annihilation are discussed. Under conditions where annihilation is absent, the two types of LHC II oligomers show distributive biexponential (bimodal) kinetics with lifetimes of 5-20 ps and 00-400 ps having transient spectra typical for chlorophyll excited states. The data can be described kinetically by a two-state compartment model involving only chlorophyll excited states. Evidence is provided that neither carotenoid excited norcarotenoid radical states are involved in the quenching mechanism at variance with earlier proposals. We propose instead that a chlorophyll-chlorophyll charge-transfer state is formed in LHC II oligomers which is an intermediate in the quenching process. The relevance to non-photochemical quenching in vivo is discussed.

Original languageEnglish
Pages (from-to)1289-1296
Number of pages8
JournalChemPhysChem
Volume11
Issue number6
DOIs
Publication statusPublished - Apr 26 2010

Fingerprint

carotenoids
Photosystem II Protein Complex
chlorophylls
Carotenoids
Chlorophyll
Energy transfer
Energy dissipation
energy dissipation
energy transfer
oligomers
Oligomers
Quenching
quenching
Excited states
excitation
Kinetics
kinetics
compartments
proposals
Charge transfer

Keywords

  • Carotenoids
  • Femtochemistry
  • Light-harvesting complex ii
  • Non-photochemical quenching
  • Photosynthesis

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Atomic and Molecular Physics, and Optics

Cite this

Singlet energy dissipation in the photosystem II light-harvesting complex does not involve energy transfer to carotenoids. / Müller, Marc G.; Lambrev, P.; Reus, Michael; Wientjes, Emilie; Croce, Roberta; Holzwarth, Alfred R.

In: ChemPhysChem, Vol. 11, No. 6, 26.04.2010, p. 1289-1296.

Research output: Contribution to journalArticle

Müller, Marc G. ; Lambrev, P. ; Reus, Michael ; Wientjes, Emilie ; Croce, Roberta ; Holzwarth, Alfred R. / Singlet energy dissipation in the photosystem II light-harvesting complex does not involve energy transfer to carotenoids. In: ChemPhysChem. 2010 ; Vol. 11, No. 6. pp. 1289-1296.
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