Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides

David Liao, P. Galajda, Robert Riehn, Rob Ilic, Jason L. Puchalla, Howard G. Yu, Harold G. Craighead, Robert H. Austin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Zero-Mode Waveguides were first introduced for Fluorescence Correlation Spectroscopy at micromolar dye concentrations. We show that combining zero-mode waveguides with fluorescence correlation spectroscopy in a continuous flow mixer avoids the compression of the FCS signal due to fluid transport at channel velocities up to ∼ 17 mm/s. We derive an analytic scaling relationship |δkON/kON| = |δkOFF/k OFF| ∼ kON+kOFF/kON 0.1D B/DF-DB e/√SNR converting this flow velocity insensitivity to improved kinetic rate certainty in time-resolved mixing experiments. Thus zero-mode waveguides make FCS suitable for direct kinetics measurements in rapid continuous flow.

Original languageEnglish
Pages (from-to)10077-10090
Number of pages14
JournalOptics Express
Volume16
Issue number14
DOIs
Publication statusPublished - Jul 7 2008

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waveguides
spectroscopy
molecules
fluorescence
deuterium fluorides
kinetics
flow velocity
dyes
scaling
fluids
sensitivity

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Liao, D., Galajda, P., Riehn, R., Ilic, R., Puchalla, J. L., Yu, H. G., ... Austin, R. H. (2008). Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides. Optics Express, 16(14), 10077-10090. https://doi.org/10.1364/OE.16.010077

Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides. / Liao, David; Galajda, P.; Riehn, Robert; Ilic, Rob; Puchalla, Jason L.; Yu, Howard G.; Craighead, Harold G.; Austin, Robert H.

In: Optics Express, Vol. 16, No. 14, 07.07.2008, p. 10077-10090.

Research output: Contribution to journalArticle

Liao, D, Galajda, P, Riehn, R, Ilic, R, Puchalla, JL, Yu, HG, Craighead, HG & Austin, RH 2008, 'Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides', Optics Express, vol. 16, no. 14, pp. 10077-10090. https://doi.org/10.1364/OE.16.010077
Liao, David ; Galajda, P. ; Riehn, Robert ; Ilic, Rob ; Puchalla, Jason L. ; Yu, Howard G. ; Craighead, Harold G. ; Austin, Robert H. / Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides. In: Optics Express. 2008 ; Vol. 16, No. 14. pp. 10077-10090.
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