Single-Channel Analysis of a Cloned Human Heart L-Type Ca2+ Channel α1 Subunit and the Effects of a Cardiac β Subunit

M. Wakamori, G. Mikala, A. Schwartz, A. Yatani

Research output: Contribution to journalArticle

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Abstract

Macroscopic and single-channel properties of a cloned human heart L-type Ca2+ channel α1 subunit expressed in Xenopus oocytes were studied and the effects of a cardiac β subunit were evaluated. The α1 subunit expressed current with much slower activation and inactivation kinetics than native cells. The β subunit increased the current amplitude and accelerated both activation and inactivation rates. Single-channel analysis revealed that the β subunit increased the probability of channel opening and shifted the voltage-dependence to more negative potentials without affecting conductance or open time. The data suggest that the β subunit modulates macroscopic current by increasing the probability of channel opening and shifting the voltage-dependence of the channel.

Original languageEnglish
Pages (from-to)1170-1176
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume196
Issue number3
DOIs
Publication statusPublished - Nov 15 1993

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Chemical activation
Electric potential
Xenopus
Oocytes
Kinetics

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Single-Channel Analysis of a Cloned Human Heart L-Type Ca2+ Channel α1 Subunit and the Effects of a Cardiac β Subunit. / Wakamori, M.; Mikala, G.; Schwartz, A.; Yatani, A.

In: Biochemical and Biophysical Research Communications, Vol. 196, No. 3, 15.11.1993, p. 1170-1176.

Research output: Contribution to journalArticle

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