Single-cell-initiated monosynaptic tracing reveals layer-specific cortical network modules

Adrian Wertz, Stuart Trenholm, Keisuke Yonehara, Daniel Hillier, Zoltan Raics, Marcus Leinweber, Gergely Szalay, Alexander Ghanem, Georg Keller, B. Rózsa, Karl Klaus Conzelmann, Botond Roska

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Individual cortical neurons can selectively respond to specific environmental features, such as visual motion or faces. How this relates to the selectivity of the presynaptic network across cortical layers remains unclear. We used single-cell - initiated, monosynaptically restricted retrograde transsynaptic tracing with rabies viruses expressing GCaMP6s to image, in vivo, the visual motion - evoked activity of individual layer 2/3 pyramidal neurons and their presynaptic networks across layers in mouse primary visual cortex. Neurons within each layer exhibited similar motion direction preferences, forming layer-specific functional modules. In one-third of the networks, the layer modules were locked to the direction preference of the postsynaptic neuron, whereas for other networks the direction preference varied by layer. Thus, there exist feature-locked and feature-variant cortical networks.

Original languageEnglish
Pages (from-to)70-74
Number of pages5
JournalScience
Volume349
Issue number6243
DOIs
Publication statusPublished - Jul 3 2015

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Neurons
Rabies virus
Pyramidal Cells
Visual Cortex
Direction compound

ASJC Scopus subject areas

  • General
  • Medicine(all)

Cite this

Wertz, A., Trenholm, S., Yonehara, K., Hillier, D., Raics, Z., Leinweber, M., ... Roska, B. (2015). Single-cell-initiated monosynaptic tracing reveals layer-specific cortical network modules. Science, 349(6243), 70-74. https://doi.org/10.1126/science.aab1687

Single-cell-initiated monosynaptic tracing reveals layer-specific cortical network modules. / Wertz, Adrian; Trenholm, Stuart; Yonehara, Keisuke; Hillier, Daniel; Raics, Zoltan; Leinweber, Marcus; Szalay, Gergely; Ghanem, Alexander; Keller, Georg; Rózsa, B.; Conzelmann, Karl Klaus; Roska, Botond.

In: Science, Vol. 349, No. 6243, 03.07.2015, p. 70-74.

Research output: Contribution to journalArticle

Wertz, A, Trenholm, S, Yonehara, K, Hillier, D, Raics, Z, Leinweber, M, Szalay, G, Ghanem, A, Keller, G, Rózsa, B, Conzelmann, KK & Roska, B 2015, 'Single-cell-initiated monosynaptic tracing reveals layer-specific cortical network modules', Science, vol. 349, no. 6243, pp. 70-74. https://doi.org/10.1126/science.aab1687
Wertz A, Trenholm S, Yonehara K, Hillier D, Raics Z, Leinweber M et al. Single-cell-initiated monosynaptic tracing reveals layer-specific cortical network modules. Science. 2015 Jul 3;349(6243):70-74. https://doi.org/10.1126/science.aab1687
Wertz, Adrian ; Trenholm, Stuart ; Yonehara, Keisuke ; Hillier, Daniel ; Raics, Zoltan ; Leinweber, Marcus ; Szalay, Gergely ; Ghanem, Alexander ; Keller, Georg ; Rózsa, B. ; Conzelmann, Karl Klaus ; Roska, Botond. / Single-cell-initiated monosynaptic tracing reveals layer-specific cortical network modules. In: Science. 2015 ; Vol. 349, No. 6243. pp. 70-74.
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