A ritkasejtes minták DNS-vizsgálatának lehetoségei, módszertana, jelentosége az ossejt-terápia szempontjából.

Translated title of the contribution: Single cell DNA analysis: possibilities, methods, implications in relation to stem cell therapy

Dominika Szoke, B. Molnár, Z. Tulassay

Research output: Contribution to journalArticle

Abstract

In the analysis of circulating tumor cells or in the preimplantation genetic diagnosis it is frequently necessary to examine one single cell. Some methods are appropriate to isolate single cells: Laser Assisted Microdissection, magnetic cell separation or FACS. The use of the whole genome amplification methods are needful, because the amount of the DNA extracted from the isolated cells is very low and inappropriate for additional examinations. With different molecular biological methods (e.g. sequencing, chip-technology) it is possible to determine genetic alterations in the analysed cells, and to return the modified cells with in vitro gene technological methods (viral vectors, non viral methods). Our aim is to summarize the methods and the possible technical problems developing during the process of the single cell molecular biological analysis.

Original languageHungarian
Pages (from-to)2183-2190
Number of pages8
JournalOrvosi Hetilap
Volume145
Issue number43
Publication statusPublished - Oct 24 2004

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Single-Cell Analysis
Cell- and Tissue-Based Therapy
Stem Cells
DNA
Preimplantation Diagnosis
Circulating Neoplastic Cells
Microdissection
Cell Separation
Lasers
Genome
Technology
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A ritkasejtes minták DNS-vizsgálatának lehetoségei, módszertana, jelentosége az ossejt-terápia szempontjából. / Szoke, Dominika; Molnár, B.; Tulassay, Z.

In: Orvosi Hetilap, Vol. 145, No. 43, 24.10.2004, p. 2183-2190.

Research output: Contribution to journalArticle

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