Simvastatin inhibits the core promoter of the TXNRD1 gene and lowers cellular TrxR activity in HepG2 cells

Lena Ekström, Maria Johansson, K. Monostory, Anna Klara Rundlöf, Elias S J Arnér, Linda Björkhem-Bergman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Thioredoxin reductase 1 (TrxR1) is a selenocysteine-containing redox-active enzyme that is thought to be important during carcinogenesis. We have recently shown that treatment with statins, HMGCoA reductase inhibitors, reduces the levels of TrxR1 in liver of both rat and human. The reduced TrxR1 levels were correlated with inhibited hepatocarcinogenesis in a rat model. The aim of the present study was to investigate if statins affect the activity of the human TXNRD1 core promoter, which guides expression of TrxR1, and if the effects by statins on TrxR1 expression in liver could be reproduced in a cellular model system. We found that simvastatin and fluvastatin decreased cellular TrxR activity in cultured human liver-derived HepG2 cells with approximately 40% (p

Original languageEnglish
Pages (from-to)90-94
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume430
Issue number1
DOIs
Publication statusPublished - Jan 4 2013

Fingerprint

Thioredoxin Reductase 1
Simvastatin
Hep G2 Cells
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Genes
Liver
fluvastatin
Rats
Selenocysteine
Human Activities
Oxidation-Reduction
Oxidoreductases
Carcinogenesis
Enzymes

Keywords

  • Fluvastatin
  • HMGCoA reductase inhibitor
  • Simvastatin
  • Thioredoxin reductase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Simvastatin inhibits the core promoter of the TXNRD1 gene and lowers cellular TrxR activity in HepG2 cells. / Ekström, Lena; Johansson, Maria; Monostory, K.; Rundlöf, Anna Klara; Arnér, Elias S J; Björkhem-Bergman, Linda.

In: Biochemical and Biophysical Research Communications, Vol. 430, No. 1, 04.01.2013, p. 90-94.

Research output: Contribution to journalArticle

Ekström, Lena ; Johansson, Maria ; Monostory, K. ; Rundlöf, Anna Klara ; Arnér, Elias S J ; Björkhem-Bergman, Linda. / Simvastatin inhibits the core promoter of the TXNRD1 gene and lowers cellular TrxR activity in HepG2 cells. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 430, No. 1. pp. 90-94.
@article{d857e6f690bf42d3b5cb5425f8fcf2ef,
title = "Simvastatin inhibits the core promoter of the TXNRD1 gene and lowers cellular TrxR activity in HepG2 cells",
abstract = "Thioredoxin reductase 1 (TrxR1) is a selenocysteine-containing redox-active enzyme that is thought to be important during carcinogenesis. We have recently shown that treatment with statins, HMGCoA reductase inhibitors, reduces the levels of TrxR1 in liver of both rat and human. The reduced TrxR1 levels were correlated with inhibited hepatocarcinogenesis in a rat model. The aim of the present study was to investigate if statins affect the activity of the human TXNRD1 core promoter, which guides expression of TrxR1, and if the effects by statins on TrxR1 expression in liver could be reproduced in a cellular model system. We found that simvastatin and fluvastatin decreased cellular TrxR activity in cultured human liver-derived HepG2 cells with approximately 40{\%} (p",
keywords = "Fluvastatin, HMGCoA reductase inhibitor, Simvastatin, Thioredoxin reductase",
author = "Lena Ekstr{\"o}m and Maria Johansson and K. Monostory and Rundl{\"o}f, {Anna Klara} and Arn{\'e}r, {Elias S J} and Linda Bj{\"o}rkhem-Bergman",
year = "2013",
month = "1",
day = "4",
doi = "10.1016/j.bbrc.2012.11.007",
language = "English",
volume = "430",
pages = "90--94",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Simvastatin inhibits the core promoter of the TXNRD1 gene and lowers cellular TrxR activity in HepG2 cells

AU - Ekström, Lena

AU - Johansson, Maria

AU - Monostory, K.

AU - Rundlöf, Anna Klara

AU - Arnér, Elias S J

AU - Björkhem-Bergman, Linda

PY - 2013/1/4

Y1 - 2013/1/4

N2 - Thioredoxin reductase 1 (TrxR1) is a selenocysteine-containing redox-active enzyme that is thought to be important during carcinogenesis. We have recently shown that treatment with statins, HMGCoA reductase inhibitors, reduces the levels of TrxR1 in liver of both rat and human. The reduced TrxR1 levels were correlated with inhibited hepatocarcinogenesis in a rat model. The aim of the present study was to investigate if statins affect the activity of the human TXNRD1 core promoter, which guides expression of TrxR1, and if the effects by statins on TrxR1 expression in liver could be reproduced in a cellular model system. We found that simvastatin and fluvastatin decreased cellular TrxR activity in cultured human liver-derived HepG2 cells with approximately 40% (p

AB - Thioredoxin reductase 1 (TrxR1) is a selenocysteine-containing redox-active enzyme that is thought to be important during carcinogenesis. We have recently shown that treatment with statins, HMGCoA reductase inhibitors, reduces the levels of TrxR1 in liver of both rat and human. The reduced TrxR1 levels were correlated with inhibited hepatocarcinogenesis in a rat model. The aim of the present study was to investigate if statins affect the activity of the human TXNRD1 core promoter, which guides expression of TrxR1, and if the effects by statins on TrxR1 expression in liver could be reproduced in a cellular model system. We found that simvastatin and fluvastatin decreased cellular TrxR activity in cultured human liver-derived HepG2 cells with approximately 40% (p

KW - Fluvastatin

KW - HMGCoA reductase inhibitor

KW - Simvastatin

KW - Thioredoxin reductase

UR - http://www.scopus.com/inward/record.url?scp=84872375099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872375099&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2012.11.007

DO - 10.1016/j.bbrc.2012.11.007

M3 - Article

C2 - 23154180

AN - SCOPUS:84872375099

VL - 430

SP - 90

EP - 94

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -