Simultaneous visualization of multiple antigens with tyramide signal amplification using antibodies from the same species

Z. Tóth, Éva Mezey

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

After immunohistochemistry (IHC) began to be used routinely, a number of investigators worked on methods for staining multiple molecules in the same tissue sections or cells. Achieving this goal was not easy, however. One reason for this is that the majority of primary antibodies used in IHC reactions are raised in rabbits, and recognizing signals from two different rabbit antibodies is not trivial. Thus, all of the protocols described to date have serious limitations. Here we report a simple, quick, and inexpensive solution to the problem. It has two major advantages over existing methods. First, by using antibodies from the same host, two or more antigens can be visualized in the same section with commercially available fluorescent dyes. Second, because the technique relies on signal amplification, both rare and abundant antigens can be detected.

Original languageEnglish
Pages (from-to)545-554
Number of pages10
JournalJournal of Histochemistry and Cytochemistry
Volume55
Issue number6
DOIs
Publication statusPublished - Jun 2007

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Antigens
Antibodies
Immunohistochemistry
Rabbits
Fluorescent Dyes
Research Personnel
Staining and Labeling

Keywords

  • Immunohistochemistry
  • Multiple antigens
  • Multiplex detection
  • Tyramide signal amplification

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

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