Simultaneous radioenzymatic assay of catecholamines and dihydroxy-phenylacetic acid /DOPAC/; comparison of the effects of drugs on the tuberoinfundibular and striatal dopamine metabolism and on plasma prolactin level

M. Fekete, B. Kanyicska, J. P. Herman

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A simultaneous radioenzymatic assay for catecholamines and DOPAC has been developed. 3H-Methoxy amines and 3H-homovanillic acid formed in the presence of 3H-S-adenosyl-methionine and catechol-O-methyl transferase were separated by organic solvent extraction and chromatography. Chlorpromazine, haloperidol, but not sulpiride increased the DOPAC content in the striatum, without affecting the DOPAC level in the median eminence. γ-Butyrolactone increased the dopamine content also only in the striatum. The monoamine oxidase inhibitor pargyline decreased the DOPAC level in both areas examined. All the substances employed increased plasma prolactin level. The biochemical responses of nigrostriatal and tubero-infundibular dopaminergic neurons may not be analogous.

Original languageEnglish
Pages (from-to)1549-1556
Number of pages8
JournalLife Sciences
Volume23
Issue number15
DOIs
Publication statusPublished - Oct 16 1978

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Corpus Striatum
3,4-Dihydroxyphenylacetic Acid
Metabolism
Prolactin
Catecholamines
Assays
Dopamine
Plasmas
Pharmaceutical Preparations
Pargyline
Guaiacol
Homovanillic Acid
Sulpiride
Median Eminence
Monoamine Oxidase Inhibitors
Dopaminergic Neurons
Chlorpromazine
Solvent extraction
Haloperidol
Transferases

ASJC Scopus subject areas

  • Pharmacology

Cite this

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abstract = "A simultaneous radioenzymatic assay for catecholamines and DOPAC has been developed. 3H-Methoxy amines and 3H-homovanillic acid formed in the presence of 3H-S-adenosyl-methionine and catechol-O-methyl transferase were separated by organic solvent extraction and chromatography. Chlorpromazine, haloperidol, but not sulpiride increased the DOPAC content in the striatum, without affecting the DOPAC level in the median eminence. γ-Butyrolactone increased the dopamine content also only in the striatum. The monoamine oxidase inhibitor pargyline decreased the DOPAC level in both areas examined. All the substances employed increased plasma prolactin level. The biochemical responses of nigrostriatal and tubero-infundibular dopaminergic neurons may not be analogous.",
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AU - Kanyicska, B.

AU - Herman, J. P.

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