Simultaneous quantitation of mono-, di- and trisaccharides as their TMS ether oxime derivatives by GC-MS: I. In model solutions

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Abstract

This paper describes the separation and quantitation of mono- di- and trisaccharides in model solutions. Optimized conditions for identification and determination of common sugar constituents in several natural matrices by GC-MS, from one solution by one injection, are given. Applying a 52 min temperature program to a 30 m capillary column and taking advantage of the stable ratios of the syn- and anti oximes eluting either alone or together with one or two other anomers: provides the basis of calculation for co- eluting compounds. Saccharides (5-200 ng), such as fructose, glucose, sucrose, trehalose, cellobiose, maltose, turanose, gentiobiose, palatinose, melibiose, isomaltose, erlose, raffinose, melezitose, maltotriose, panose and isomaltotriose, in the presence of each other in various ratios, have been measured as silyloxime compounds with a reproducibility of ≤ 5 R.S.D. %. The possibility of measuring all these saccharides as their TMS methoximes is also presented.

Original languageEnglish
Pages (from-to)321-327
Number of pages7
JournalChromatographia
Volume45
DOIs
Publication statusPublished - 1997

Fingerprint

Trisaccharides
Oximes
Disaccharides
Ether
Isomaltose
Melibiose
Raffinose
Derivatives
Cellobiose
Trehalose
Maltose
Fructose
Sugars
Sucrose
Identification (control systems)
Glucose
Injections
Temperature
glucosylsucrose
gentiobiose

Keywords

  • Gas chromatography-mass spectrometry
  • Mono-, di- and trisaccharides
  • Syn/anti oxime distribution
  • Trimethylsilyl oximes(methoximes)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

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title = "Simultaneous quantitation of mono-, di- and trisaccharides as their TMS ether oxime derivatives by GC-MS: I. In model solutions",
abstract = "This paper describes the separation and quantitation of mono- di- and trisaccharides in model solutions. Optimized conditions for identification and determination of common sugar constituents in several natural matrices by GC-MS, from one solution by one injection, are given. Applying a 52 min temperature program to a 30 m capillary column and taking advantage of the stable ratios of the syn- and anti oximes eluting either alone or together with one or two other anomers: provides the basis of calculation for co- eluting compounds. Saccharides (5-200 ng), such as fructose, glucose, sucrose, trehalose, cellobiose, maltose, turanose, gentiobiose, palatinose, melibiose, isomaltose, erlose, raffinose, melezitose, maltotriose, panose and isomaltotriose, in the presence of each other in various ratios, have been measured as silyloxime compounds with a reproducibility of ≤ 5 R.S.D. {\%}. The possibility of measuring all these saccharides as their TMS methoximes is also presented.",
keywords = "Gas chromatography-mass spectrometry, Mono-, di- and trisaccharides, Syn/anti oxime distribution, Trimethylsilyl oximes(methoximes)",
author = "I. Moln{\'a}r-Perl and K. Horv{\'a}th",
year = "1997",
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journal = "Chromatographia",
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N2 - This paper describes the separation and quantitation of mono- di- and trisaccharides in model solutions. Optimized conditions for identification and determination of common sugar constituents in several natural matrices by GC-MS, from one solution by one injection, are given. Applying a 52 min temperature program to a 30 m capillary column and taking advantage of the stable ratios of the syn- and anti oximes eluting either alone or together with one or two other anomers: provides the basis of calculation for co- eluting compounds. Saccharides (5-200 ng), such as fructose, glucose, sucrose, trehalose, cellobiose, maltose, turanose, gentiobiose, palatinose, melibiose, isomaltose, erlose, raffinose, melezitose, maltotriose, panose and isomaltotriose, in the presence of each other in various ratios, have been measured as silyloxime compounds with a reproducibility of ≤ 5 R.S.D. %. The possibility of measuring all these saccharides as their TMS methoximes is also presented.

AB - This paper describes the separation and quantitation of mono- di- and trisaccharides in model solutions. Optimized conditions for identification and determination of common sugar constituents in several natural matrices by GC-MS, from one solution by one injection, are given. Applying a 52 min temperature program to a 30 m capillary column and taking advantage of the stable ratios of the syn- and anti oximes eluting either alone or together with one or two other anomers: provides the basis of calculation for co- eluting compounds. Saccharides (5-200 ng), such as fructose, glucose, sucrose, trehalose, cellobiose, maltose, turanose, gentiobiose, palatinose, melibiose, isomaltose, erlose, raffinose, melezitose, maltotriose, panose and isomaltotriose, in the presence of each other in various ratios, have been measured as silyloxime compounds with a reproducibility of ≤ 5 R.S.D. %. The possibility of measuring all these saccharides as their TMS methoximes is also presented.

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KW - Syn/anti oxime distribution

KW - Trimethylsilyl oximes(methoximes)

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