Simultaneous presence of neutrophil alveolitis and Ki-67 positivity of alveolar macrophages in dermato-/polymyositis and systemic sclerosis

Gábor Kumánovics, T. Magyarlaki, A. Komócsi, György Szekeres, L. Czirják

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: The proportion of cell proliferation-associated nuclear Ki-67 antigen positive alveolar macrophages was compared in the bronchoalveolar lavage fluids (BAL) of patients with dermato-/polymyositis (DM-PM), scleroderma-myositis overlap syndrome (Scl-M), systemic sclerosis (SSc), and undifferentiated connective tissue disease (UCTD). Patients and methods: We examined 8 patients with DM-PM, 3 with Scl-M, 11 with SSc, and 10 with UCTD. The patients were selected on the basis of their interstitial lung involvement diagnosed by non-invasive methods. Results: Increased Ki-67 expression in alveolar macrophages was observed in three cases of SSc (27%) and in 4 of myositis (36%). In UCTD and in healthy volunteers a low level of Ki-67 expression was detected (in both groups 5%). A positive correlation was found between the percentage of the Ki-67 positive alveolar macrophages and the percentage of granulocytes in BAL fluid of the investigated patients with different systemic autoimmune diseases and healthy controls, indicating that increased local proliferation of differentiated large alveolar macrophages can be detected in cases with neutrophil alveolitis. There was also a correlation between the extent of ground glass opacity on HRCT and elevated proportion of proliferating alveolar macrophages in BAL fluid.

Original languageEnglish
Pages (from-to)6-10
Number of pages5
JournalRheumatology International
Volume23
Issue number1
Publication statusPublished - Jan 2003

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Polymyositis
Systemic Scleroderma
Alveolar Macrophages
Neutrophils
Myositis
Connective Tissue Diseases
Bronchoalveolar Lavage Fluid
Ki-67 Antigen
Nuclear Antigens
Granulocytes
Autoimmune Diseases
Glass
Healthy Volunteers
Cell Proliferation
Lung

Keywords

  • Alveolar macrophage
  • Alveolitis
  • Bronchoalveolar lavage
  • Connective tissue diseases
  • Ki-67

ASJC Scopus subject areas

  • Rheumatology

Cite this

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title = "Simultaneous presence of neutrophil alveolitis and Ki-67 positivity of alveolar macrophages in dermato-/polymyositis and systemic sclerosis",
abstract = "Objective: The proportion of cell proliferation-associated nuclear Ki-67 antigen positive alveolar macrophages was compared in the bronchoalveolar lavage fluids (BAL) of patients with dermato-/polymyositis (DM-PM), scleroderma-myositis overlap syndrome (Scl-M), systemic sclerosis (SSc), and undifferentiated connective tissue disease (UCTD). Patients and methods: We examined 8 patients with DM-PM, 3 with Scl-M, 11 with SSc, and 10 with UCTD. The patients were selected on the basis of their interstitial lung involvement diagnosed by non-invasive methods. Results: Increased Ki-67 expression in alveolar macrophages was observed in three cases of SSc (27{\%}) and in 4 of myositis (36{\%}). In UCTD and in healthy volunteers a low level of Ki-67 expression was detected (in both groups 5{\%}). A positive correlation was found between the percentage of the Ki-67 positive alveolar macrophages and the percentage of granulocytes in BAL fluid of the investigated patients with different systemic autoimmune diseases and healthy controls, indicating that increased local proliferation of differentiated large alveolar macrophages can be detected in cases with neutrophil alveolitis. There was also a correlation between the extent of ground glass opacity on HRCT and elevated proportion of proliferating alveolar macrophages in BAL fluid.",
keywords = "Alveolar macrophage, Alveolitis, Bronchoalveolar lavage, Connective tissue diseases, Ki-67",
author = "G{\'a}bor Kum{\'a}novics and T. Magyarlaki and A. Kom{\'o}csi and Gy{\"o}rgy Szekeres and L. Czirj{\'a}k",
year = "2003",
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journal = "Rheumatology International",
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T1 - Simultaneous presence of neutrophil alveolitis and Ki-67 positivity of alveolar macrophages in dermato-/polymyositis and systemic sclerosis

AU - Kumánovics, Gábor

AU - Magyarlaki, T.

AU - Komócsi, A.

AU - Szekeres, György

AU - Czirják, L.

PY - 2003/1

Y1 - 2003/1

N2 - Objective: The proportion of cell proliferation-associated nuclear Ki-67 antigen positive alveolar macrophages was compared in the bronchoalveolar lavage fluids (BAL) of patients with dermato-/polymyositis (DM-PM), scleroderma-myositis overlap syndrome (Scl-M), systemic sclerosis (SSc), and undifferentiated connective tissue disease (UCTD). Patients and methods: We examined 8 patients with DM-PM, 3 with Scl-M, 11 with SSc, and 10 with UCTD. The patients were selected on the basis of their interstitial lung involvement diagnosed by non-invasive methods. Results: Increased Ki-67 expression in alveolar macrophages was observed in three cases of SSc (27%) and in 4 of myositis (36%). In UCTD and in healthy volunteers a low level of Ki-67 expression was detected (in both groups 5%). A positive correlation was found between the percentage of the Ki-67 positive alveolar macrophages and the percentage of granulocytes in BAL fluid of the investigated patients with different systemic autoimmune diseases and healthy controls, indicating that increased local proliferation of differentiated large alveolar macrophages can be detected in cases with neutrophil alveolitis. There was also a correlation between the extent of ground glass opacity on HRCT and elevated proportion of proliferating alveolar macrophages in BAL fluid.

AB - Objective: The proportion of cell proliferation-associated nuclear Ki-67 antigen positive alveolar macrophages was compared in the bronchoalveolar lavage fluids (BAL) of patients with dermato-/polymyositis (DM-PM), scleroderma-myositis overlap syndrome (Scl-M), systemic sclerosis (SSc), and undifferentiated connective tissue disease (UCTD). Patients and methods: We examined 8 patients with DM-PM, 3 with Scl-M, 11 with SSc, and 10 with UCTD. The patients were selected on the basis of their interstitial lung involvement diagnosed by non-invasive methods. Results: Increased Ki-67 expression in alveolar macrophages was observed in three cases of SSc (27%) and in 4 of myositis (36%). In UCTD and in healthy volunteers a low level of Ki-67 expression was detected (in both groups 5%). A positive correlation was found between the percentage of the Ki-67 positive alveolar macrophages and the percentage of granulocytes in BAL fluid of the investigated patients with different systemic autoimmune diseases and healthy controls, indicating that increased local proliferation of differentiated large alveolar macrophages can be detected in cases with neutrophil alveolitis. There was also a correlation between the extent of ground glass opacity on HRCT and elevated proportion of proliferating alveolar macrophages in BAL fluid.

KW - Alveolar macrophage

KW - Alveolitis

KW - Bronchoalveolar lavage

KW - Connective tissue diseases

KW - Ki-67

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