Simultaneous non-instrumental detection of aflatoxin B1 and ochratoxin A using a clean-up tandem immunoassay column

Irina Yu Goryacheva, Sarah De Saeger, Barbara Delmulle, Marieke Lobeau, Sergei A. Eremin, I. Barna-Vetró, Carlos Van Peteghem

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

A set-up and simple method based on the clean-up tandem immunoassay approach was developed for the visual detection of two analytes. The method was based on a 1 mL column with one clean-up layer and two detection immunolayers. As detection immunolayers CNBr-activated Sepharose 4B with coupled secondary rabbit anti-mouse antibodies was used. Different specific antibodies were coupled to each detection immunolayer. The analysis was realised in a competitive ELISA format with visual detection of the developed colour for each detection immunolayer and took 20 min for six sample extracts. The described method was applied to the simultaneous detection of aflatoxin B1 and ochratoxin A in spices with cut-off levels at 5 and 10 μg kg-1, respectively. Results were confirmed by LC-MS/MS with immunoaffinity column clean-up.

Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalAnalytica Chimica Acta
Volume590
Issue number1
DOIs
Publication statusPublished - May 2 2007

Fingerprint

Aflatoxin B1
immunoassay
Immunoassay
Antibodies
Sepharose
Spices
Color
Anti-Idiotypic Antibodies
antibody
Enzyme-Linked Immunosorbent Assay
Rabbits
spice
ochratoxin A
detection
method

Keywords

  • Aflatoxin B1
  • Immunoassay
  • Multiple analysis
  • Mycotoxins
  • Ochratoxin A
  • Rapid methods
  • Spices

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Simultaneous non-instrumental detection of aflatoxin B1 and ochratoxin A using a clean-up tandem immunoassay column. / Goryacheva, Irina Yu; De Saeger, Sarah; Delmulle, Barbara; Lobeau, Marieke; Eremin, Sergei A.; Barna-Vetró, I.; Van Peteghem, Carlos.

In: Analytica Chimica Acta, Vol. 590, No. 1, 02.05.2007, p. 118-124.

Research output: Contribution to journalArticle

Goryacheva, Irina Yu ; De Saeger, Sarah ; Delmulle, Barbara ; Lobeau, Marieke ; Eremin, Sergei A. ; Barna-Vetró, I. ; Van Peteghem, Carlos. / Simultaneous non-instrumental detection of aflatoxin B1 and ochratoxin A using a clean-up tandem immunoassay column. In: Analytica Chimica Acta. 2007 ; Vol. 590, No. 1. pp. 118-124.
@article{f8eab23f0bfa49dcbc7587236445aa57,
title = "Simultaneous non-instrumental detection of aflatoxin B1 and ochratoxin A using a clean-up tandem immunoassay column",
abstract = "A set-up and simple method based on the clean-up tandem immunoassay approach was developed for the visual detection of two analytes. The method was based on a 1 mL column with one clean-up layer and two detection immunolayers. As detection immunolayers CNBr-activated Sepharose 4B with coupled secondary rabbit anti-mouse antibodies was used. Different specific antibodies were coupled to each detection immunolayer. The analysis was realised in a competitive ELISA format with visual detection of the developed colour for each detection immunolayer and took 20 min for six sample extracts. The described method was applied to the simultaneous detection of aflatoxin B1 and ochratoxin A in spices with cut-off levels at 5 and 10 μg kg-1, respectively. Results were confirmed by LC-MS/MS with immunoaffinity column clean-up.",
keywords = "Aflatoxin B1, Immunoassay, Multiple analysis, Mycotoxins, Ochratoxin A, Rapid methods, Spices",
author = "Goryacheva, {Irina Yu} and {De Saeger}, Sarah and Barbara Delmulle and Marieke Lobeau and Eremin, {Sergei A.} and I. Barna-Vetr{\'o} and {Van Peteghem}, Carlos",
year = "2007",
month = "5",
day = "2",
doi = "10.1016/j.aca.2007.02.075",
language = "English",
volume = "590",
pages = "118--124",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Simultaneous non-instrumental detection of aflatoxin B1 and ochratoxin A using a clean-up tandem immunoassay column

AU - Goryacheva, Irina Yu

AU - De Saeger, Sarah

AU - Delmulle, Barbara

AU - Lobeau, Marieke

AU - Eremin, Sergei A.

AU - Barna-Vetró, I.

AU - Van Peteghem, Carlos

PY - 2007/5/2

Y1 - 2007/5/2

N2 - A set-up and simple method based on the clean-up tandem immunoassay approach was developed for the visual detection of two analytes. The method was based on a 1 mL column with one clean-up layer and two detection immunolayers. As detection immunolayers CNBr-activated Sepharose 4B with coupled secondary rabbit anti-mouse antibodies was used. Different specific antibodies were coupled to each detection immunolayer. The analysis was realised in a competitive ELISA format with visual detection of the developed colour for each detection immunolayer and took 20 min for six sample extracts. The described method was applied to the simultaneous detection of aflatoxin B1 and ochratoxin A in spices with cut-off levels at 5 and 10 μg kg-1, respectively. Results were confirmed by LC-MS/MS with immunoaffinity column clean-up.

AB - A set-up and simple method based on the clean-up tandem immunoassay approach was developed for the visual detection of two analytes. The method was based on a 1 mL column with one clean-up layer and two detection immunolayers. As detection immunolayers CNBr-activated Sepharose 4B with coupled secondary rabbit anti-mouse antibodies was used. Different specific antibodies were coupled to each detection immunolayer. The analysis was realised in a competitive ELISA format with visual detection of the developed colour for each detection immunolayer and took 20 min for six sample extracts. The described method was applied to the simultaneous detection of aflatoxin B1 and ochratoxin A in spices with cut-off levels at 5 and 10 μg kg-1, respectively. Results were confirmed by LC-MS/MS with immunoaffinity column clean-up.

KW - Aflatoxin B1

KW - Immunoassay

KW - Multiple analysis

KW - Mycotoxins

KW - Ochratoxin A

KW - Rapid methods

KW - Spices

UR - http://www.scopus.com/inward/record.url?scp=34047151951&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34047151951&partnerID=8YFLogxK

U2 - 10.1016/j.aca.2007.02.075

DO - 10.1016/j.aca.2007.02.075

M3 - Article

C2 - 17416231

AN - SCOPUS:34047151951

VL - 590

SP - 118

EP - 124

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

IS - 1

ER -