Simultaneous LC determination of ergosterol, tocopherols and carotenoids in foods

Hussein G. Daood, Margit Korbász, Samer Hamdan, Judit Beczner

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A simple and reliable liquid chromatographic method was worked out to simultaneously determine ergosterol, as biomarker of fungal contamination, tocopherols and carotenoids as important phytochemicals having antioxidant properties in foods. The method developed in the present work included alkaline hydrolysis of the food sample, solvent extraction, chromatographic separation and photodiode-array detection. The separation was performed on the newest generation of Zorbax reversed-phase adsorbent with extra dense bonding of organo-silane ligands with double end-capping. Gradient elution system starting with water containing methanol and ending with a mixture of methanol- isopropanol-acetonitrile was used. The separated compounds were detected by using photodiode-array detection at wavelength ranges of 190-700 nm with quantification accomplished at the maximum absorbance wavelength for each analyte. The method was validated on the basis of recovery, precision, linearity of the calibration curves, and limit of detection and quantification using standard materials for ergosterol, α-tocopherol and β-carotene. The method was successfully applied for the determination of important phytochemicals in some spice and herbal products. The lowest detectable limit of such constituents was between 0.1 and 0.5 μg g-1 food. Positive correlation was not found between the ergosterol content and fungal count number of spice red pepper subjected to thermal drying and ambient storage.

Original languageEnglish
Pages (from-to)S137-S140
JournalChromatographia
Volume68
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Oct 1 2008

Keywords

  • Carotenoids
  • Column liquid chromatography
  • Paprika
  • Tocopherols and ergosterol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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