Simultaneous evaluation of viability and acrosome integrity of mouse spermatozoa using light microscopy

T. Somfai, Sz Bodó, E. Gócza, J. Iváncsics, A. Kovács

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Determination of the percentage of live cells with intact acrosomes and no morphologic aberrations could be a practical index of semen quality. We applied viability and acrosome staining techniques, originally described for bull, boar and rabbit sperm, to mouse spermatozoa. The viability stain was either trypan blue or Congo red. The stain was precipitated by neutral red in the fixative. The acrosome was stained by Giemsa. Sperm morphology, including cytoplasmic droplets, could be evaluated as well. The staining method described here is a useful routine tool for simultaneous evaluation of the plasma membrane integrity of different sperm subdomains, the status of the acrosome, and cellular morphology.

Original languageEnglish
Pages (from-to)117-120
Number of pages4
JournalBiotechnic and Histochemistry
Volume77
Issue number3
Publication statusPublished - 2002

Fingerprint

Acrosome
Spermatozoa
Microscopy
Light
Coloring Agents
Staining and Labeling
Neutral Red
Congo Red
Fixatives
Semen Analysis
Trypan Blue
Cell Membrane
Rabbits

Keywords

  • Acrosome integrity
  • Light microscopy
  • Mouse
  • Spermatozoa
  • Viability

ASJC Scopus subject areas

  • Anatomy
  • Applied Microbiology and Biotechnology

Cite this

Simultaneous evaluation of viability and acrosome integrity of mouse spermatozoa using light microscopy. / Somfai, T.; Bodó, Sz; Gócza, E.; Iváncsics, J.; Kovács, A.

In: Biotechnic and Histochemistry, Vol. 77, No. 3, 2002, p. 117-120.

Research output: Contribution to journalArticle

Somfai, T. ; Bodó, Sz ; Gócza, E. ; Iváncsics, J. ; Kovács, A. / Simultaneous evaluation of viability and acrosome integrity of mouse spermatozoa using light microscopy. In: Biotechnic and Histochemistry. 2002 ; Vol. 77, No. 3. pp. 117-120.
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