Simultaneous effect of organic modifier and physicochemical parameters of barbiturates on their retention on a narrow-bore PGC column

E. Forgács, T. Cserháti, Ivan Miksik, Adam Echardt, Zdenek Deyl

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The retention time of 22 barbituric acid derivatives was measured on a narrow-bore porous graphitized carbon (PGC) column using water-dioxane mixtures as mobile phases. The capacity factor (k), theoretical plate number (N), and asymmetry factor (AF) were calculated for each solute in each mobile phase. The relationships between chromatographic characteristics and physicochemical parameters of solutes were elucidated by stepwise regression analysis (SRA). SRA indicated that the binding of barbiturates to the PGC surface is of mixed character electrostatic and apolar interactive forces are equally involved. Sterical correspondence between the surface of the stationary phase and the solutes also exert a significant influence on the retention behavior.

Original languageEnglish
Pages (from-to)259-262
Number of pages4
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume800
Issue number1-2
DOIs
Publication statusPublished - Feb 5 2004

Fingerprint

Barbiturates
Regression analysis
Carbon
Regression Analysis
Static Electricity
Electrostatics
Derivatives
Water
1,4-dioxane
barbituric acid

Keywords

  • Barbiturates
  • Porous graphitized carbon

ASJC Scopus subject areas

  • Biochemistry

Cite this

@article{01748bafc6d448478b6f4774c96410f3,
title = "Simultaneous effect of organic modifier and physicochemical parameters of barbiturates on their retention on a narrow-bore PGC column",
abstract = "The retention time of 22 barbituric acid derivatives was measured on a narrow-bore porous graphitized carbon (PGC) column using water-dioxane mixtures as mobile phases. The capacity factor (k), theoretical plate number (N), and asymmetry factor (AF) were calculated for each solute in each mobile phase. The relationships between chromatographic characteristics and physicochemical parameters of solutes were elucidated by stepwise regression analysis (SRA). SRA indicated that the binding of barbiturates to the PGC surface is of mixed character electrostatic and apolar interactive forces are equally involved. Sterical correspondence between the surface of the stationary phase and the solutes also exert a significant influence on the retention behavior.",
keywords = "Barbiturates, Porous graphitized carbon",
author = "E. Forg{\'a}cs and T. Cserh{\'a}ti and Ivan Miksik and Adam Echardt and Zdenek Deyl",
year = "2004",
month = "2",
day = "5",
doi = "10.1016/j.jchromb.2003.10.050",
language = "English",
volume = "800",
pages = "259--262",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Simultaneous effect of organic modifier and physicochemical parameters of barbiturates on their retention on a narrow-bore PGC column

AU - Forgács, E.

AU - Cserháti, T.

AU - Miksik, Ivan

AU - Echardt, Adam

AU - Deyl, Zdenek

PY - 2004/2/5

Y1 - 2004/2/5

N2 - The retention time of 22 barbituric acid derivatives was measured on a narrow-bore porous graphitized carbon (PGC) column using water-dioxane mixtures as mobile phases. The capacity factor (k), theoretical plate number (N), and asymmetry factor (AF) were calculated for each solute in each mobile phase. The relationships between chromatographic characteristics and physicochemical parameters of solutes were elucidated by stepwise regression analysis (SRA). SRA indicated that the binding of barbiturates to the PGC surface is of mixed character electrostatic and apolar interactive forces are equally involved. Sterical correspondence between the surface of the stationary phase and the solutes also exert a significant influence on the retention behavior.

AB - The retention time of 22 barbituric acid derivatives was measured on a narrow-bore porous graphitized carbon (PGC) column using water-dioxane mixtures as mobile phases. The capacity factor (k), theoretical plate number (N), and asymmetry factor (AF) were calculated for each solute in each mobile phase. The relationships between chromatographic characteristics and physicochemical parameters of solutes were elucidated by stepwise regression analysis (SRA). SRA indicated that the binding of barbiturates to the PGC surface is of mixed character electrostatic and apolar interactive forces are equally involved. Sterical correspondence between the surface of the stationary phase and the solutes also exert a significant influence on the retention behavior.

KW - Barbiturates

KW - Porous graphitized carbon

UR - http://www.scopus.com/inward/record.url?scp=0348047454&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0348047454&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2003.10.050

DO - 10.1016/j.jchromb.2003.10.050

M3 - Article

VL - 800

SP - 259

EP - 262

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 1-2

ER -