Simultaneous effect of organic modifier and physicochemical parameters of some steroidal drugs on their retention on a narrow-bore C1 Column. Part 2

Research output: Contribution to journalArticle

Abstract

The retention of 13 steroidal drugs was determined on a narrow-bore C1 column; the mobile phases being mixtures of water-methanol mixtures and the retention factor (k) was calculated for each mobile phase and drug. The relationships among the chromatographic parameters, mobile phase composition, and physicochemical parameters of solutes were elucidated by principal component analysis followed by two-dimensional non-linear mapping and varimax rotation around two axes. Computations indicated that the binding of steroidal drugs to the surface of the stationary phase is of mixed character: electrostatic and polar interactive forces are equally involved. Sterical correspondence between the surface of the stationary phase and the solutes also exerts a considerable influence on the retention behavior. The comparison of results with results obtained by employing other sets of steroidal drugs indicated the marked effect of the character of substituents on the results of QSRR computations.

Original languageEnglish
Pages (from-to)2481-2492
Number of pages12
JournalJournal of Liquid Chromatography and Related Technologies
Volume35
Issue number17
DOIs
Publication statusPublished - Jan 1 2012

    Fingerprint

Keywords

  • Narrow-bore column
  • Organic malifiers
  • Physicichemical parameters
  • Steroids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

Cite this