Simultaneous determination of organic acids and sugars in apples by gas-liquid chromatography

Magdolna Morvai, Ibolya Molnár-Perl

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A simple and rapid gas-liquid chromatographic method for the simultaneous determination of aliphatic mono-, di- and tricarboxylic acids, cyclic acids (shikimic + quinic), aromatic acids and sugars, all as trimethylsilyl derivatives in a single solution, was developed. Optimum conditions for the simultaneous derivatization and for the quantitative evaluation of organic acids and sugars were established. Reproductibility data showed that 0.25-1 μg of the characteristic acids of apples in the presence of a 700-2500-fold excess of sugars can be determined with a relative standard deviation (R.S.D.) of 10.7% or less. The main components (malic acid, fructose, glucose and sucrose) were determined with an R.S.D. of 4.0% or less.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalJournal of Chromatography A
Volume520
Issue numberC
DOIs
Publication statusPublished - Nov 9 1990

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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