Simultaneous determination of dextran and glucose in serum

A. Rédei, S. Nagy

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

IN the course of an investigation on the mechanism of 'anaphylactoid' reaction in rats, the need was felt for an accurate and simple procedure for the simultaneous determination of dextran and glucose in small amounts of serum. For this purpose the following method was developed. It utilizes the o-toluidin - glucose coupling reaction1, producing a green colour suitable for colorimetric measurement. Dextran is precipitated from the serum by the o-toluidin - acetic acid reagent followed by hydrolysis and the colour reaction. Serum glucose is directly determined from the supernatant layer of the dextran precipitate.

Original languageEnglish
Pages (from-to)173-174
Number of pages2
JournalNature
Volume191
Issue number4784
DOIs
Publication statusPublished - Dec 1 1961

ASJC Scopus subject areas

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