Simultaneous detection of X- and Y-bearing bull spermatozoa by double colour fluorescence in situ hybridization

Mohamed Hassanane, Andras Kovacs, Pascal Laurent, Kerstin Lindblad, Ingemar Gustavsson

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Double colour fluorescence in situ hybridization with sex chromosome probes was applied on sperm cells of fice Swedish Holstein-Friesian bulls. It was demonstrated that cosmids with strong fluorecence signals and scraped chromosomes can successfully be used as markers in this type of study. X and Y segregated as expected according to a 1:1 ratio, and there were no interindividual variations. There was a tendency for there to be more Y- than X-bearing spermatozoa, but this bias was assumed to be due to the markers used. Disomic spermatozoa occurred with a frequency of more than 0.1% (0.067% XX, 0.029% YY, and 0.029% XY), which is considerably lower than the frequency in humans. Diploid sperm cells occurred with a frequency of 0.05%.

Original languageEnglish
Pages (from-to)407-412
Number of pages6
JournalMolecular Reproduction and Development
Volume53
Issue number4
DOIs
Publication statusPublished - Jul 14 1999

Keywords

  • Bull
  • FISH
  • Nondisjunction
  • Spermatozoa
  • X chromosome
  • Y chromosome

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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