Simultaneous detection of viability and sex of bovine spermatozoa

T. Révay, A. Kovács, W. Rens, I. Gustavsson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The viability and sex of bovine spermatozoa were simultaneously evaluated. After viability and acrosome staining with trypan blue/Giemsa, only live spermatozoa became decondensed by a modified papain-dithiothreitol method. Owing to this specific effect, live sperm heads were easily distinguished by their enlarged size and dark violet colour from small, light blue dead sperm heads. In the same sperm sample, X- and Y-chromosome-bearing sperm were distinguished by their fluorescent signal, using fluorescence in situ hybridization (FISH) with an XY paint set and 4,6-diamino-2-phenylindole counterstaining. The combined staining provides a method for morphological and viability evaluation before FISH and permits identification of the proportions of X- and Y-chromosome-containing live spermatozoa in a semen sample. However, only 25% of the undecondensed dead sperm express signals allowing detection of the sex of the chromosome. The method may be an effective tool in evaluating sex-oriented semen samples.

Original languageEnglish
Pages (from-to)373-376
Number of pages4
JournalReproduction, Fertility and Development
Volume14
Issue number5-6
Publication statusPublished - 2002

Fingerprint

Spermatozoa
spermatozoa
viability
gender
cattle
Sperm Head
Semen
Fluorescence In Situ Hybridization
Y chromosome
X chromosome
fluorescence in situ hybridization
semen
Staining and Labeling
Acrosome
Sex Chromosomes
Paint
Papain
Trypan Blue
Dithiothreitol
Y Chromosome

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine
  • Developmental Biology
  • Endocrinology

Cite this

Simultaneous detection of viability and sex of bovine spermatozoa. / Révay, T.; Kovács, A.; Rens, W.; Gustavsson, I.

In: Reproduction, Fertility and Development, Vol. 14, No. 5-6, 2002, p. 373-376.

Research output: Contribution to journalArticle

Révay, T. ; Kovács, A. ; Rens, W. ; Gustavsson, I. / Simultaneous detection of viability and sex of bovine spermatozoa. In: Reproduction, Fertility and Development. 2002 ; Vol. 14, No. 5-6. pp. 373-376.
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