Simultaneous detection of three procine viruses by multiplex PCR

L. Sámi, K. Ursu, J. McKillen, S. Kecskeméti, S. Belák, István Kiss

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Specific oligonucleotide primers were selected and combined in a multiplex arrangement, in order to detect simultaneously three economically important porcine viruses by polymerase chain reaction (PCR). The pathogen panel was comprised of viruses that cause reproductive failure in infected herds: Aujeszky.s disease virus (ADV), porcine parvovirus (PPV) and porcine respiratory and reproductive syndrome virus (PRRSV). In order to reduce the time required for the detection of the pathogens, the assay was optimised to a RapidCycler PCR instrument. The multiplex PCR assay was shown to be specific, sensitive and rapid, because the results were read in less than 60 min after sample preparation. Due to its speed, efficiency and sensitivity, the described rapid multiplex PCR assay serves as a useful novel tool in the veterinary diagnostic laboratories for the quick and complex detection of these important porcine pathogens.

Original languageEnglish
Pages (from-to)267-276
Number of pages10
JournalActa Veterinaria Hungarica
Volume55
Issue number2
DOIs
Publication statusPublished - Jun 2007

Fingerprint

Multiplex Polymerase Chain Reaction
Swine
Porcine Parvovirus
polymerase chain reaction
Porcine respiratory and reproductive syndrome virus
Viruses
Polymerase Chain Reaction
viruses
DNA Primers
Virus Diseases
assays
microbial detection
Ungulate protoparvovirus 1
swine
pathogens
Porcine reproductive and respiratory syndrome virus
DNA primers
herds
sampling

Keywords

  • ADV
  • Multiplex PCR
  • PPV
  • PRRSV
  • Rapid PCR

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Simultaneous detection of three procine viruses by multiplex PCR. / Sámi, L.; Ursu, K.; McKillen, J.; Kecskeméti, S.; Belák, S.; Kiss, István.

In: Acta Veterinaria Hungarica, Vol. 55, No. 2, 06.2007, p. 267-276.

Research output: Contribution to journalArticle

Sámi, L. ; Ursu, K. ; McKillen, J. ; Kecskeméti, S. ; Belák, S. ; Kiss, István. / Simultaneous detection of three procine viruses by multiplex PCR. In: Acta Veterinaria Hungarica. 2007 ; Vol. 55, No. 2. pp. 267-276.
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