Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV-2) were detected simultaneously with immunohistological (IH) examination in lungs of 5 growing pigs died of pneumonia, came from a large scale farm. Histological lesions characteristic to PCV-2 infection (occurrence of multinucleated giant cells) was observed only in 2 cases. Histological lesions correspond to secondary bacterial infection were always evident together with viral interstitial pneumonia. Pathogenic bacteria were isolated in three cases from the lungs (Pasteurella multocida in two cases. Streptococcus spp. in one case). In two cases PCV-2 could not be observed in all five tissue samples of cranial part of the lungs, which were investigated in each animal, and in most of the cases the amount of virus-antigen showed great variance in the different tissue samples of the same animal. PRRSV was demonstrated in each tissue samples and mostly in large amount with IH. PCV-2 and PRRSV were evident consistently in different cells, and simultaneous occurrence of the two viruses in the same cell was never observed, using the double IH method. This is the first report about the detection of PRRSV with IH in Hungary, and it is also the first time of the demonstration of simultaneous occurrence of PRRSV and PCV-2 in lungs of swine died of pneumonia in this country.
|Number of pages||8|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - Dec 1 2007|
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