Simple high-performance liquid chromatographic method for the determination of tolbutamide and its metabolites in human plasma and urine using photodiode-array detection

Katalin Csillag, László Vereczkey, Béla Gachályi

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Abstract

A high-performance liquid chromatographic method has been developed for the determination of tolbutamide and its metabolites in human plasma and urine. The compounds examined were extracted with diethyl ether from the acidified biological fluid. Chlorpropamide was used as internal standard, and 235 nm was chosen as the wavelength for diode-array detection. A study of the relationship between the capacity factor and the mobile phase composition and pH showed that acetonitrile-2-propanol-0.1% orthophosphoric acid (17:17:66, v/v) was the best eluent on a C8 reversed-phase column. The method is precise, sensitive and suitable for pharmacological investigations.

Original languageEnglish
Pages (from-to)355-363
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume490
Issue numberC
DOIs
Publication statusPublished - 1989

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ASJC Scopus subject areas

  • Chemistry(all)

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