Simple detection method for distinguishing dead and living yeast colonies

J. Kucsera, Kyoko Yarita, Kanji Takeo

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

A rapid and simple assay was developed for detection of yeast colonies containing dying or dead cells. Methylene blue, phloxin B, rose bengal and trypan blue at concentrations of 5-10 μmol l-1 were shown to stain non-viable cells in colonies of Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans and Filobasidium capsuligenum without staining or affecting the viability of living cells of the colonies. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)19-21
Number of pages3
JournalJournal of Microbiological Methods
Volume41
Issue number1
DOIs
Publication statusPublished - 2000

Fingerprint

Eosine I Bluish
Yeasts
Rose Bengal
Trypan Blue
Schizosaccharomyces
Methylene Blue
Candida albicans
Saccharomyces cerevisiae
Cell Survival
Coloring Agents
Staining and Labeling

Keywords

  • Dead cells
  • Dye uptake
  • Phloxin B

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Simple detection method for distinguishing dead and living yeast colonies. / Kucsera, J.; Yarita, Kyoko; Takeo, Kanji.

In: Journal of Microbiological Methods, Vol. 41, No. 1, 2000, p. 19-21.

Research output: Contribution to journalArticle

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