Simple correction improving long-term reproducibility of HPLC-MS

Eszter Tõth, Helga Hevér, Olivér Ozohanics, András Telekes, K. Vékey, L. Drahos

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Chromatographic peak areas in long series of high-performance liquid chromatography-MS experiments often vary, which decrease reproducibility and may cause bias in the results. It was found that the sensitivity of various components change differently; in our case, variability is in the order of 20-40%, and it is most likely due to changing conditions in electrospray ionization (ESI). The most often used peak area correction methods do not take this effect into account. The change in peak areas can be well described by a polynomial function; we found that a fourth-order polynomial is most often suitable. We suggest a simple correction algorithm based on polynomial fitting. When the experiments were inherently well reproducible, this correction improved reproducibility from 12% to 3% (on average for various components). When random errors were larger, this improvement was less significant (15% to 12% in nano-ESI) but nevertheless essential in order to avoid possible bias in the results.

Original languageEnglish
Pages (from-to)1130-1135
Number of pages6
JournalJournal of Mass Spectrometry
Volume50
Issue number10
DOIs
Publication statusPublished - Oct 1 2015

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Electrospray ionization
polynomials
High Pressure Liquid Chromatography
Polynomials
ionization
Random errors
random errors
liquid chromatography
High performance liquid chromatography
Experiments
causes
sensitivity

Keywords

  • electrospray
  • HPLC-MS
  • long-term stability
  • proteomics
  • signal drift

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Simple correction improving long-term reproducibility of HPLC-MS. / Tõth, Eszter; Hevér, Helga; Ozohanics, Olivér; Telekes, András; Vékey, K.; Drahos, L.

In: Journal of Mass Spectrometry, Vol. 50, No. 10, 01.10.2015, p. 1130-1135.

Research output: Contribution to journalArticle

Tõth, Eszter ; Hevér, Helga ; Ozohanics, Olivér ; Telekes, András ; Vékey, K. ; Drahos, L. / Simple correction improving long-term reproducibility of HPLC-MS. In: Journal of Mass Spectrometry. 2015 ; Vol. 50, No. 10. pp. 1130-1135.
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