Simple and rapid determination of clomiphene cis and trans isomers in human plasma by high-performance liquid chromatography using on-line post-column photochemical derivatization and fluorescence detection

I. Ürmös, S. M. Benkö, I. Klebovich

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A validated, sensitive and rapid high-performance high-performance liquid chromatographic method has been developed for the analysis of both cis and trans isomers of clomiphene in human plasma. The method involves a new off-line pre-column solid-phase extraction for sample preparation of clomiphene from human plasma in the presence of an internal standard. Analysis was performed by isocratic elution on a LiChrospher 100 RP-18 5-μm column using on-line post-column photochemical derivatization, with fluorescence detection at 247 nm (excitation) and 378 nm (emission). The limit of quantitation was 0.75 ng/ml for the cis isomer and 1.25 ng/ml for the trans isomer. The precision and accuracy of method were between good laboratory practice (GLP) required limits.

Original languageEnglish
Pages (from-to)168-172
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume617
Issue number1
DOIs
Publication statusPublished - Jul 23 1993

Fingerprint

Enclomiphene
Plasma (human)
Clomiphene
High performance liquid chromatography
Isomers
Fluorescence
High Pressure Liquid Chromatography
Solid Phase Extraction
Liquids

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

@article{2b68ad6a38e24c96bc29927e7cbee34d,
title = "Simple and rapid determination of clomiphene cis and trans isomers in human plasma by high-performance liquid chromatography using on-line post-column photochemical derivatization and fluorescence detection",
abstract = "A validated, sensitive and rapid high-performance high-performance liquid chromatographic method has been developed for the analysis of both cis and trans isomers of clomiphene in human plasma. The method involves a new off-line pre-column solid-phase extraction for sample preparation of clomiphene from human plasma in the presence of an internal standard. Analysis was performed by isocratic elution on a LiChrospher 100 RP-18 5-μm column using on-line post-column photochemical derivatization, with fluorescence detection at 247 nm (excitation) and 378 nm (emission). The limit of quantitation was 0.75 ng/ml for the cis isomer and 1.25 ng/ml for the trans isomer. The precision and accuracy of method were between good laboratory practice (GLP) required limits.",
author = "I. {\"U}rm{\"o}s and Benk{\"o}, {S. M.} and I. Klebovich",
year = "1993",
month = "7",
day = "23",
doi = "10.1016/0378-4347(93)80438-A",
language = "English",
volume = "617",
pages = "168--172",
journal = "Journal of Chromatography B: Biomedical Sciences and Applications",
issn = "0378-4347",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Simple and rapid determination of clomiphene cis and trans isomers in human plasma by high-performance liquid chromatography using on-line post-column photochemical derivatization and fluorescence detection

AU - Ürmös, I.

AU - Benkö, S. M.

AU - Klebovich, I.

PY - 1993/7/23

Y1 - 1993/7/23

N2 - A validated, sensitive and rapid high-performance high-performance liquid chromatographic method has been developed for the analysis of both cis and trans isomers of clomiphene in human plasma. The method involves a new off-line pre-column solid-phase extraction for sample preparation of clomiphene from human plasma in the presence of an internal standard. Analysis was performed by isocratic elution on a LiChrospher 100 RP-18 5-μm column using on-line post-column photochemical derivatization, with fluorescence detection at 247 nm (excitation) and 378 nm (emission). The limit of quantitation was 0.75 ng/ml for the cis isomer and 1.25 ng/ml for the trans isomer. The precision and accuracy of method were between good laboratory practice (GLP) required limits.

AB - A validated, sensitive and rapid high-performance high-performance liquid chromatographic method has been developed for the analysis of both cis and trans isomers of clomiphene in human plasma. The method involves a new off-line pre-column solid-phase extraction for sample preparation of clomiphene from human plasma in the presence of an internal standard. Analysis was performed by isocratic elution on a LiChrospher 100 RP-18 5-μm column using on-line post-column photochemical derivatization, with fluorescence detection at 247 nm (excitation) and 378 nm (emission). The limit of quantitation was 0.75 ng/ml for the cis isomer and 1.25 ng/ml for the trans isomer. The precision and accuracy of method were between good laboratory practice (GLP) required limits.

UR - http://www.scopus.com/inward/record.url?scp=0027290918&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027290918&partnerID=8YFLogxK

U2 - 10.1016/0378-4347(93)80438-A

DO - 10.1016/0378-4347(93)80438-A

M3 - Article

C2 - 8376533

AN - SCOPUS:0027290918

VL - 617

SP - 168

EP - 172

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

SN - 0378-4347

IS - 1

ER -