Simple and rapid determination of clomiphene cis and trans isomers in human plasma by high-performance liquid chromatography using on-line post-column photochemical derivatization and fluorescence detection

I. Ürmös, S. M. Benkö, I. Klebovich

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A validated, sensitive and rapid high-performance high-performance liquid chromatographic method has been developed for the analysis of both cis and trans isomers of clomiphene in human plasma. The method involves a new off-line pre-column solid-phase extraction for sample preparation of clomiphene from human plasma in the presence of an internal standard. Analysis was performed by isocratic elution on a LiChrospher 100 RP-18 5-μm column using on-line post-column photochemical derivatization, with fluorescence detection at 247 nm (excitation) and 378 nm (emission). The limit of quantitation was 0.75 ng/ml for the cis isomer and 1.25 ng/ml for the trans isomer. The precision and accuracy of method were between good laboratory practice (GLP) required limits.

Original languageEnglish
Pages (from-to)168-172
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume617
Issue number1
DOIs
Publication statusPublished - Jul 23 1993

ASJC Scopus subject areas

  • Chemistry(all)

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