Simple and rapid detection of carnation etched ring virus by polymerase chain reaction

L. Palkovics, E. Balázs

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Polymerase chain reaction was used to identify carnation etched ring virus (CERV) in leaves of infected carnations. Two 20-mer oligonucleotide primers were designed to the conserved intergenic sequence of carnation etched ring virus. The 850 nt amplified fragment was cloned and sequenced, showing 95.6% identity with the published sequence. The cloned fragment was used after radioactive labeling to prove the viral origin of the PCR products amplified from nucleic acid samples originating from plant extracts. Different DNA extraction methods were compared and the alkaline extraction method was chosen, due to its simplicity to screen large numbers of samples.

Original languageEnglish
Pages (from-to)161-168
Number of pages8
JournalActa Phytopathologica et Entomologica Hungarica
Volume31
Issue number3-4
Publication statusPublished - 1996

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Carnation etched ring virus
polymerase chain reaction
Dianthus caryophyllus
DNA primers
intergenic DNA
plant extracts
nucleic acids
sampling
DNA
methodology
leaves

ASJC Scopus subject areas

  • Insect Science
  • Plant Science

Cite this

Simple and rapid detection of carnation etched ring virus by polymerase chain reaction. / Palkovics, L.; Balázs, E.

In: Acta Phytopathologica et Entomologica Hungarica, Vol. 31, No. 3-4, 1996, p. 161-168.

Research output: Contribution to journalArticle

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