Polymerase chain reaction was used to identify carnation etched ring virus (CERV) in leaves of infected carnations. Two 20-mer oligonucleotide primers were designed to the conserved intergenic sequence of carnation etched ring virus. The 850 nt amplified fragment was cloned and sequenced, showing 95.6% identity with the published sequence. The cloned fragment was used after radioactive labeling to prove the viral origin of the PCR products amplified from nucleic acid samples originating from plant extracts. Different DNA extraction methods were compared and the alkaline extraction method was chosen, due to its simplicity to screen large numbers of samples.
|Number of pages||8|
|Journal||Acta Phytopathologica et Entomologica Hungarica|
|Publication status||Published - Dec 1 1996|
ASJC Scopus subject areas
- Plant Science
- Insect Science