Simple and Efficient Method for Isolation and Measurement of Cyanobacterial Hepatotoxins by Plant Tests (Sinapis alba L.)

P. Kós, G. Gorzo, G. Suranyi, G. Borbély

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

A simple and cost-effective method for isolating and assaying microcystins, cyanobacterial toxins, by C-18 cartridges, DEAE-cellulose (DE-52) chromatography, and a mustard (Sinapis alba L.) plant seedling test is described. The procedure results in a purity of up to 95-97% microcystin without the need for an HPLC system and justifies the use of the S. alba L. seedling test in the quantitative assessment of the toxin with an IC50 of 3 μg ml-1 instead of the mouse intraperitoneal test.

Original languageEnglish
Pages (from-to)49-53
Number of pages5
JournalAnalytical Biochemistry
Volume225
Issue number1
DOIs
Publication statusPublished - Feb 1995

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Sinapis
Microcystins
DEAE-Cellulose
Chromatography
Seedlings
Mustard Plant
Inhibitory Concentration 50
Costs
High Pressure Liquid Chromatography
Costs and Cost Analysis
microcystin
cyanobacterial toxin

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

Simple and Efficient Method for Isolation and Measurement of Cyanobacterial Hepatotoxins by Plant Tests (Sinapis alba L.). / Kós, P.; Gorzo, G.; Suranyi, G.; Borbély, G.

In: Analytical Biochemistry, Vol. 225, No. 1, 02.1995, p. 49-53.

Research output: Contribution to journalArticle

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