Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells

Katalin Kerti-Szigeti, Z. Nusser

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Hippocampal pyramidal cells (PCs) express many GABAAR subunit types and receive GABAergic inputs from distinct interneurons. Previous experiments revealed input-specific differences in α1 and α2 subunit densities in perisomatic synapses, suggesting distinct IPSC decay kinetics. However, IPSC decays evoked by axo-axonic, parvalbumin- or cholecystokinin-expressing basket cells were found to be similar. Using replica immunogold labeling, here we show that all CA1 PC somatic and AIS synapses contain the α1, α2, β1, β2, β3 and γ2 subunits. In CA3 PCs, 90% of the perisomatic synapses are immunopositive for the α1 subunit and all synapses are positive for the remaining five subunits. Somatic synapses form unimodal distributions based on their immunoreactivity for these subunits. The α2 subunit densities in somatic synapses facing Cav2.1 (i.e. parvalbumin) or Cav2.2 (cholecystokinin) positive presynaptic active zones are comparable. We conclude that perisomatic synapses made by three distinct interneuron types have similar GABAA receptor subunit content.

Original languageEnglish
Article numbere18426
JournaleLife
Volume5
Issue numberAUGUST
DOIs
Publication statusPublished - Aug 18 2016

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Parvalbumins
Pyramidal Cells
Cholecystokinin
GABA-A Receptors
Synapses
Chemical analysis
Labeling
Interneurons
Kinetics
Experiments
Axons
Axon Initial Segment
voltage-dependent calcium channel (P-Q type)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells. / Kerti-Szigeti, Katalin; Nusser, Z.

In: eLife, Vol. 5, No. AUGUST, e18426, 18.08.2016.

Research output: Contribution to journalArticle

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