Silymarin and vitamin E reduce amiodarone-induced lysosomal phospholipidosis in rats

Márta Ágoston, Ferenc Örsi, E. Fehér, K. Hagymási, Zsuzsa Orosz, A. Blázovics, J. Fehér, András Vereckei

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Several antioxidants have been shown to reduce lysosomal phospholipidosis, which is a potential mechanism of amiodarone toxicity, and prevent amiodarone toxicity by antioxidant and/or non-antioxidant mechanisms. The aim of this study was to test whether the co-administration of two structurally different antioxidants vitamin E and silymarin with amiodarone can reduce amiodarone-induced lysosomal phospholipidosis, and if yes, by reducing the tissue concentration of amiodarone and desethylamiodarone or by their antioxidant action. To this end, male Fischer 344 rats were treated by gavage once a day for 3 weeks and randomly assigned to the following four experimental groups: 1, control; 2, amiodarone (150 mg/(kg per day)); 3, amiodarone (150 mg/(kg per day)) plus vitamin E (100 mg/(kg per day)); 4, amiodarone (150 mg/(kg per day)) plus silymarin (60 mg/(kg per day)) treated groups. Total plasma phospholipid (PL), liver-conjugated diene, thiobarbituric acid reactive substances (TBARSs), amiodarone and desethylamiodarone concentrations were determined and the extent of lysosomal phospholipidosis in the liver was estimated by a semi-quantitative electron microscopic method. Amiodarone treatment increased significantly the liver-conjugated diene (P

Original languageEnglish
Pages (from-to)231-241
Number of pages11
JournalToxicology
Volume190
Issue number3
DOIs
Publication statusPublished - Aug 28 2003

Fingerprint

Silymarin
Amiodarone
Vitamin E
Rats
Antioxidants
Liver
Toxicity
Thiobarbituric Acid Reactive Substances
Inbred F344 Rats
Phospholipids
Electrons
Tissue

Keywords

  • Amiodarone toxicity
  • Lysosomal phospholipidosis
  • Silymarin
  • Vitamin E

ASJC Scopus subject areas

  • Toxicology

Cite this

Silymarin and vitamin E reduce amiodarone-induced lysosomal phospholipidosis in rats. / Ágoston, Márta; Örsi, Ferenc; Fehér, E.; Hagymási, K.; Orosz, Zsuzsa; Blázovics, A.; Fehér, J.; Vereckei, András.

In: Toxicology, Vol. 190, No. 3, 28.08.2003, p. 231-241.

Research output: Contribution to journalArticle

Ágoston, Márta ; Örsi, Ferenc ; Fehér, E. ; Hagymási, K. ; Orosz, Zsuzsa ; Blázovics, A. ; Fehér, J. ; Vereckei, András. / Silymarin and vitamin E reduce amiodarone-induced lysosomal phospholipidosis in rats. In: Toxicology. 2003 ; Vol. 190, No. 3. pp. 231-241.
@article{d19bfb37ddb647ca95f4e7fff8c2a4ec,
title = "Silymarin and vitamin E reduce amiodarone-induced lysosomal phospholipidosis in rats",
abstract = "Several antioxidants have been shown to reduce lysosomal phospholipidosis, which is a potential mechanism of amiodarone toxicity, and prevent amiodarone toxicity by antioxidant and/or non-antioxidant mechanisms. The aim of this study was to test whether the co-administration of two structurally different antioxidants vitamin E and silymarin with amiodarone can reduce amiodarone-induced lysosomal phospholipidosis, and if yes, by reducing the tissue concentration of amiodarone and desethylamiodarone or by their antioxidant action. To this end, male Fischer 344 rats were treated by gavage once a day for 3 weeks and randomly assigned to the following four experimental groups: 1, control; 2, amiodarone (150 mg/(kg per day)); 3, amiodarone (150 mg/(kg per day)) plus vitamin E (100 mg/(kg per day)); 4, amiodarone (150 mg/(kg per day)) plus silymarin (60 mg/(kg per day)) treated groups. Total plasma phospholipid (PL), liver-conjugated diene, thiobarbituric acid reactive substances (TBARSs), amiodarone and desethylamiodarone concentrations were determined and the extent of lysosomal phospholipidosis in the liver was estimated by a semi-quantitative electron microscopic method. Amiodarone treatment increased significantly the liver-conjugated diene (P",
keywords = "Amiodarone toxicity, Lysosomal phospholipidosis, Silymarin, Vitamin E",
author = "M{\'a}rta {\'A}goston and Ferenc {\"O}rsi and E. Feh{\'e}r and K. Hagym{\'a}si and Zsuzsa Orosz and A. Bl{\'a}zovics and J. Feh{\'e}r and Andr{\'a}s Vereckei",
year = "2003",
month = "8",
day = "28",
doi = "10.1016/S0300-483X(03)00188-4",
language = "English",
volume = "190",
pages = "231--241",
journal = "Toxicology",
issn = "0300-483X",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Silymarin and vitamin E reduce amiodarone-induced lysosomal phospholipidosis in rats

AU - Ágoston, Márta

AU - Örsi, Ferenc

AU - Fehér, E.

AU - Hagymási, K.

AU - Orosz, Zsuzsa

AU - Blázovics, A.

AU - Fehér, J.

AU - Vereckei, András

PY - 2003/8/28

Y1 - 2003/8/28

N2 - Several antioxidants have been shown to reduce lysosomal phospholipidosis, which is a potential mechanism of amiodarone toxicity, and prevent amiodarone toxicity by antioxidant and/or non-antioxidant mechanisms. The aim of this study was to test whether the co-administration of two structurally different antioxidants vitamin E and silymarin with amiodarone can reduce amiodarone-induced lysosomal phospholipidosis, and if yes, by reducing the tissue concentration of amiodarone and desethylamiodarone or by their antioxidant action. To this end, male Fischer 344 rats were treated by gavage once a day for 3 weeks and randomly assigned to the following four experimental groups: 1, control; 2, amiodarone (150 mg/(kg per day)); 3, amiodarone (150 mg/(kg per day)) plus vitamin E (100 mg/(kg per day)); 4, amiodarone (150 mg/(kg per day)) plus silymarin (60 mg/(kg per day)) treated groups. Total plasma phospholipid (PL), liver-conjugated diene, thiobarbituric acid reactive substances (TBARSs), amiodarone and desethylamiodarone concentrations were determined and the extent of lysosomal phospholipidosis in the liver was estimated by a semi-quantitative electron microscopic method. Amiodarone treatment increased significantly the liver-conjugated diene (P

AB - Several antioxidants have been shown to reduce lysosomal phospholipidosis, which is a potential mechanism of amiodarone toxicity, and prevent amiodarone toxicity by antioxidant and/or non-antioxidant mechanisms. The aim of this study was to test whether the co-administration of two structurally different antioxidants vitamin E and silymarin with amiodarone can reduce amiodarone-induced lysosomal phospholipidosis, and if yes, by reducing the tissue concentration of amiodarone and desethylamiodarone or by their antioxidant action. To this end, male Fischer 344 rats were treated by gavage once a day for 3 weeks and randomly assigned to the following four experimental groups: 1, control; 2, amiodarone (150 mg/(kg per day)); 3, amiodarone (150 mg/(kg per day)) plus vitamin E (100 mg/(kg per day)); 4, amiodarone (150 mg/(kg per day)) plus silymarin (60 mg/(kg per day)) treated groups. Total plasma phospholipid (PL), liver-conjugated diene, thiobarbituric acid reactive substances (TBARSs), amiodarone and desethylamiodarone concentrations were determined and the extent of lysosomal phospholipidosis in the liver was estimated by a semi-quantitative electron microscopic method. Amiodarone treatment increased significantly the liver-conjugated diene (P

KW - Amiodarone toxicity

KW - Lysosomal phospholipidosis

KW - Silymarin

KW - Vitamin E

UR - http://www.scopus.com/inward/record.url?scp=0042133190&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042133190&partnerID=8YFLogxK

U2 - 10.1016/S0300-483X(03)00188-4

DO - 10.1016/S0300-483X(03)00188-4

M3 - Article

C2 - 12927377

AN - SCOPUS:0042133190

VL - 190

SP - 231

EP - 241

JO - Toxicology

JF - Toxicology

SN - 0300-483X

IS - 3

ER -